najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМО10.17586/2220-8054-2024-15-3-352-360najo-104Research ArticleCHEMISTRY AND MATERIALS SCIENCEХИМИЯ И НАУКА О МАТЕРИАЛАХCellular uptake of FITC-labeled Ce0/8Gd0/2O2-x nanoparticles in 2D and 3D mesenchymal stem cell systemsПроникновение наночастиц ФИТС-меченных наночастиц Ce0.8Gd0.2O2-x в 2D и 3D клеточные системы на основе мезенхимальных стволовых клетокhttps://orcid.org/0000-0003-3391-7889КолмановичД. Д.KolmanovichD. D.

Данил Денисович Колманович

Пушкино

Danil D. Kolmanovich

Pushchino

kdd100996@mail.ruhttps://orcid.org/0000-0001-8431-4485ЧукавинН. Н.ChukavinN. N.

Никита Николаевич Чукавин

Пушкино; Москва

Nikita N. Chukavin

Pushchino; Moscow

chukavinnik@gmail.comhttps://orcid.org/0009-0005-1997-0845ПивоваровН. А.PivovarovN. A.

Никита Артемович Пивоваров

Пушкино

Nikita A. Pivovarov

Pushchino; Moscow

nikitapivovarov.workmail@gmail.comhttps://orcid.org/0000-0001-9286-3644ХаустовС. А.KhaustovS. A.

Сергей Анатольевич Хаустов

Москва

Sergey A. Khaustov

Moscow

sergeykhaustov@gmail.comhttps://orcid.org/0000-0003-2343-2140ИвановВ. К.IvanovV. K.

Владимир Константинович Иванов

Москва

Vladimir K. Ivanov

Moscow

van@igic.ras.ruhttps://orcid.org/0000-0003-2643-4846ПоповА. Л.PopovA. L.

Антон Леонидович Попов

Пушкино; Москва

Anton L. Popov

Pushchino; Moscow

antonpopovleonid@gmail.comИнститут теоретической и экспериментальной биофизики Российской академии наукInstitute of Theoretical and Experimental Biophysics of the Russian Academy of SciencesИнститут теоретической и экспериментальной биофизики Российской академии наук; Научно-образовательный центр Государственного педагогического университетаInstitute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences; Scientific and Educational Center, State University of EducationИнститут теоретической и экспериментальной биофизики Российской академии наукInstitute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences; Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of SciencesНаучно-образовательный центр Государственного педагогического университетаScientific and Educational Center, State University of EducationИнститут общей и неорганической химии им. Н.С. Курнакова РАНKurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences202401062025153352360Copyright © Kolmanovich D.D., Chukavin N.N., Pivovarov N.A., Khaustov S.A., Ivanov V.K., Popov A.L., 20252025Колманович Д.Д., Чукавин Н.Н., Пивоваров Н.А., Хаустов С.А., Иванов В.К., Попов А.Л.Kolmanovich D.D., Chukavin N.N., Pivovarov N.A., Khaustov S.A., Ivanov V.K., Popov A.L.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/104

      Cerium-containing nanoparticles have recently been identified as promising nanozymes for advanced biomedical applications. Additional modification of the core or the surface of CeO2 nanoparticles (CeO2 NPs) provides them with new functionalities, making them a unique theranostic agent. In this study, dextran-stabilized CeO2 NPs doped with Gd (Ce0.8Gd0.2O2−x) were synthesized and further functionalized with fluorescein isothiocyanate (FITC). The synthesized nanoparticles have a high degree of biocompatibility at concentrations up to 5 mg/mL and are readily internalized by human mesenchymal stem cells cultured both in monolayers (2D system) and cellular spheroids (3D system). The functionalization of CeO2 NPs with Gd and FITC dye allows for monitoring their accumulation within organs and tissues using both magnetic resonance imaging (MRI) and fluorescence spectroscopy techniques.

   Церий-содержащие наночастицы являются перспективными нанозимами для передовых биомедицинских применений. Дополнительная модификация ядра или поверхности наночастиц CeO2 придает им новые функциональные возможности, что делает их уникальным тераностическим средством. В этом исследовании синтезировали декстран-стабилизированные наночастицы CeO2, легированные Gd, и дополнительно функционализировали их флуоресцеин изотиоцианатом (ФИТС). Синтезированные наночастицы обладают высокой степенью биосовместимости при концентрациях до 5 мг/мл и легко проникают в мезенхимальные стволовые клетки человека, культивируемыми как в монослое (2D-система), так и в клеточных сфероидах (3D-система). Функционализация наночастиц CeO2 с помощью ионов гадолиния и или ФИТС-метки позволяет контролировать их накопление в органах и тканях с помощью методов как магнитно-резонансной томографии (МРТ), так и флуоресцентной спектроскопии.

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The authors declare that there are no conflicts of interest present.