najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМОnajo-1077Research ArticlePHYSICSФИЗИКАMeasuring local quantum yield of photoluminescence and phototransformations with laser scanning microscopeMeasuring local quantum yield of photoluminescence and phototransformations with laser scanning microscopeZakharovV. V.ZakharovV. V.

St. Petersburg

St. Petersburg

Viktor-zah@yandex.ruBaranovM. A.BaranovM. A.

St. Petersburg

St. Petersburg

mbaranov@mail.ruZlatovA. S.ZlatovA. S.

St. Petersburg

St. Petersburg

zlatov.andrei@gmail.comVeniaminovA. V.VeniaminovA. V.

St. Petersburg

St. Petersburg

veniaminov@phoi.ifmo.ruITMO University; Optec LLCITMO University; Optec LLCITMO UniversityITMO University20141708202556811819Copyright © Zakharov V.V., Baranov M.A., Zlatov A.S., Veniaminov A.V., 20252025Zakharov V.V., Baranov M.A., Zlatov A.S., Veniaminov A.V.Zakharov V.V., Baranov M.A., Zlatov A.S., Veniaminov A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/1077

   Measurement of local quantum yields for the photoluminescence of semiconductor nanocrystals (quantum dots) and photoinduced transformations of dye molecules in polymer films is demonstrated using a laser scanning microscope capable of mapping luminescence spectra and intensities of transmitted laser light. The confocal scanning microscope (Zeiss LSM710) was applied for both the induction of photochemical transformations and measurement The luminescence quantum yield values for quantum dots in different locations of a polymer film were found to differ, ostensibly depending on their aggregation. To measure photoisomerization quantum yield, the effects of scanning a tiny area of a polymer film with a focused beam on the intensities of luminescence and transmitted light were monitored.

   Measurement of local quantum yields for the photoluminescence of semiconductor nanocrystals (quantum dots) and photoinduced transformations of dye molecules in polymer films is demonstrated using a laser scanning microscope capable of mapping luminescence spectra and intensities of transmitted laser light. The confocal scanning microscope (Zeiss LSM710) was applied for both the induction of photochemical transformations and measurement The luminescence quantum yield values for quantum dots in different locations of a polymer film were found to differ, ostensibly depending on their aggregation. To measure photoisomerization quantum yield, the effects of scanning a tiny area of a polymer film with a focused beam on the intensities of luminescence and transmitted light were monitored.

confocal microscopyluminescencephotoisomerizationquantum dotthioindigoconfocal microscopyluminescencephotoisomerizationquantum dotthioindigoThe authors gratefully acknowledge the financial support of this work from the Ministry of Education and Science of the Russian Federation (Grant No. 14.B25.31.0002) and the Russian Foundation for Basic Research (Grants No. 12-02-01263 and No. 12-02-00938)The authors gratefully acknowledge the financial support of this work from the Ministry of Education and Science of the Russian Federation (Grant No. 14.B25.31.0002) and the Russian Foundation for Basic Research (Grants No. 12-02-01263 and No. 12-02-00938)ReferencesRajeshwar K., de Tacconi N.R., Chenthamarakshan C.R. Semiconductor-Based Composite Materials: Preparation, Properties, and Performance. Chem. Mater., 13, P. 2765–2782 (2001).Rajeshwar K., de Tacconi N.R., Chenthamarakshan C.R. Semiconductor-Based Composite Materials: Preparation, Properties, and Performance. Chem. Mater., 13, P. 2765–2782 (2001).Tomczak N., Janyczewski D., Han M., Vancso G.J. Designer polymer-quantum dot architectures. Progr. Polymer Sci., 34, P. 393–430 (2009).Tomczak N., Janyczewski D., Han M., Vancso G.J. Designer polymer-quantum dot architectures. Progr. Polymer Sci., 34, P. 393–430 (2009).Bukowski T.J., Simmons J.H. Quantum Dot Research: Current State and Future Prospects. Critical Reviews in Solid State and Materials Sciences, P. 119–142 (2002).Bukowski T.J., Simmons J.H. Quantum Dot Research: Current State and Future Prospects. Critical Reviews in Solid State and Materials Sciences, P. 119–142 (2002).Demas J.N., Crosby G.A. The Measurement of Photoluminescence Quantum Yields. Review, J. Phys. Chem., 75, P. 991–1024 (1971).Demas J.N., Crosby G.A. The Measurement of Photoluminescence Quantum Yields. Review, J. Phys. Chem., 75, P. 991–1024 (1971).Williams A.T.R., Winfield S.A., Miller J.N. Relative fluorescence quantum yields using a computer controlled luminescence spectrometer. Analyst, 108, P. 1067–1071 (1983).Williams A.T.R., Winfield S.A., Miller J.N. Relative fluorescence quantum yields using a computer controlled luminescence spectrometer. Analyst, 108, P. 1067–1071 (1983).Lakowicz J.R. Principles of Fluorescence Spectroscopy, 3<sup>rd</sup> ed. Springer, 954 p. (2006).Lakowicz J.R. Principles of Fluorescence Spectroscopy, 3<sup>rd</sup> ed. Springer, 954 p. (2006).Carlson L., Krauss T.D. Direct measurement of the fluorescence quantum yield for individual single-walled carbon nanotubes. Proc. Conf. Electrochem. Soc. ‘Carbon Nanotubes and Nanostructures: Fundamental Properties and Processes’, Abstr. 1018 (2007).Carlson L., Krauss T.D. Direct measurement of the fluorescence quantum yield for individual single-walled carbon nanotubes. Proc. Conf. Electrochem. Soc. ‘Carbon Nanotubes and Nanostructures: Fundamental Properties and Processes’, Abstr. 1018 (2007).Brouwer A.M. Standards for photoluminescence quantum yield measurements in solution (IUPAC Technical Report). Pure Appl. Chem., 83, P. 2213–2228 (2011).Brouwer A.M. Standards for photoluminescence quantum yield measurements in solution (IUPAC Technical Report). Pure Appl. Chem., 83, P. 2213–2228 (2011).Wuerth C., Grabolle M., et al. Relative and absolute determination of fluorescence quantum yields of transparent samples. Nature Protocols, 8, P. 1535–1550 (2013).Wuerth C., Grabolle M., et al. Relative and absolute determination of fluorescence quantum yields of transparent samples. Nature Protocols, 8, P. 1535–1550 (2013).Kuhn H.J., Braslavsky S.E., Schmidt R. Chemical Actinometry (IUPAC Technical Report). Pure Appl. Chem., 76, P. 2105–2146 (2004).Kuhn H.J., Braslavsky S.E., Schmidt R. Chemical Actinometry (IUPAC Technical Report). Pure Appl. Chem., 76, P. 2105–2146 (2004).Gaigalas A.K., Wang L. Measurement of the fluorescence quantum yield using a spectrometer with an integrating sphere detector. J. Res. Natl. Inst. Stand. Technol., 113, P. 17–28 (2008).Gaigalas A.K., Wang L. Measurement of the fluorescence quantum yield using a spectrometer with an integrating sphere detector. J. Res. Natl. Inst. Stand. Technol., 113, P. 17–28 (2008).Bindhu C.V., Harilal S.S., et al. Measurement of the absolute fluorescence quantum yield of rhodamine B solution using a dual-beam thermal lens technique. J. Phys. D: Appl. Phys., 29, P. 1074–1079 (1996).Bindhu C.V., Harilal S.S., et al. Measurement of the absolute fluorescence quantum yield of rhodamine B solution using a dual-beam thermal lens technique. J. Phys. D: Appl. Phys., 29, P. 1074–1079 (1996).Ebenstein Y., Mokari T., Banina U. Fluorescence quantum yield of CdSe/ZnS nanocrystals investigated by correlated atomic-force and single-particle fluorescence microscopy. Appl. Phys. Lett., 80, P. 4033 (2002).Ebenstein Y., Mokari T., Banina U. Fluorescence quantum yield of CdSe/ZnS nanocrystals investigated by correlated atomic-force and single-particle fluorescence microscopy. Appl. Phys. Lett., 80, P. 4033 (2002).Mostoslavskii M.A. Photochromic Thioindigoid Dyes. In: Organic Photochromes, ed. by Eltsov A.V., Springer, New York and London, P. 45–104 (1990).Mostoslavskii M.A. Photochromic Thioindigoid Dyes. In: Organic Photochromes, ed. by Eltsov A.V., Springer, New York and London, P. 45–104 (1990).Lashkov G.I., Popov A.P., Ratner O.B. Three-dimensional phase-recording reoxan media with physical development of a latent image. Opt. Spectrosc., 52, P. 350–352 (1982).Lashkov G.I., Popov A.P., Ratner O.B. Three-dimensional phase-recording reoxan media with physical development of a latent image. Opt. Spectrosc., 52, P. 350–352 (1982).P.A. Carapellucci, H.P. Wolf, K. Weiss. Photoreduction of 9,10-Phenanthrenequinone. J. Amer. Chem. Soc., 91, P. 4635–4639 (1969).P.A. Carapellucci, H.P. Wolf, K. Weiss. Photoreduction of 9,10-Phenanthrenequinone. J. Amer. Chem. Soc., 91, P. 4635–4639 (1969).Veniaminov A.V., Mahilny U.V. Holographic Polymer Materials with Diffusion Development: Principles, Arrangement, Investigation, and Applications. Opt. Spectrosc., 115, P. 902–925 (2013).Veniaminov A.V., Mahilny U.V. Holographic Polymer Materials with Diffusion Development: Principles, Arrangement, Investigation, and Applications. Opt. Spectrosc., 115, P. 902–925 (2013).Haucke G., Paetzold R. Photophysikalische Chemie Indigoider Farbstoffe. Nova Acta Leopoldina. Deutsche Akademie der Naturforscher Leopoldina, Halle (Saale), 123 p. (1978).Haucke G., Paetzold R. Photophysikalische Chemie Indigoider Farbstoffe. Nova Acta Leopoldina. Deutsche Akademie der Naturforscher Leopoldina, Halle (Saale), 123 p. (1978).Veniaminov A.V., Lashkov G.I. Photochemical isomerization of thioindigo derivatives embedded in poly(methylmethacrylate). Polymer Sci. USSR, 28, P. 963–971 (1986).Veniaminov A.V., Lashkov G.I. Photochemical isomerization of thioindigo derivatives embedded in poly(methylmethacrylate). Polymer Sci. USSR, 28, P. 963–971 (1986).

The authors declare that there are no conflicts of interest present.