najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМО10.17586/2220-8054-2016-7-4-774-779najo-1360Research ArticleСтатьиIn-situ functionalization of aniline oligomer onto layered graphene sheet and study of its application on electrochemical detection of ascorbic acidin food samplesIn-situ functionalization of aniline oligomer onto layered graphene sheet and study of its application on electrochemical detection of ascorbic acidin food samplesDevasenaS.DevasenaS.

Department of Chemistry

Madurai–625009

Department of Chemistry

Madurai–625009

MeenakshiS.MeenakshiS.

Department of Inorganic Chemistry

Guindy, Chennai–600025

Department of Inorganic Chemistry

Guindy, Chennai–600025

 

SayeekannanR.SayeekannanR.

Department of Chemistry

Madurai–625009

Department of Chemistry

Madurai–625009

PandianK.PandianK.

Department of Inorganic Chemistry

Guindy, Chennai–600025

Department of Inorganic Chemistry

Guindy, Chennai–600025

jeevapandian@yahoo.co.ukThiagarajar CollegeИндияThiagarajar CollegeIndiaUniversity of MadrasИндияUniversity of MadrasIndia20162208202574774779Copyright © Devasena S., Meenakshi S., Sayeekannan R., Pandian K., 20252025Devasena S., Meenakshi S., Sayeekannan R., Pandian K.Devasena S., Meenakshi S., Sayeekannan R., Pandian K.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/1360

Aniline oligomers are considered as one of the electron transfer mediators for the electrochemical oxidation of ascorbic acid. The electrochemical oxidation of ascorbic acid was investigated using aniline oligomer-functionalized polymer modified electrode. In the present investigation, we demonstrated a novel methodology for the in-situ modification of aniline oligomer onto the layered graphene sheet by using diazonium salt form as precursor molecule. An enhanced electrocatalytic current was obtained for the oxidation of ascorbic acid using aniline pentamer-functionalized reduced graphene oxide (AP-rGO). Detailed studies have been carried out to study the surface modified rGO by FTIR spectroscopy. A linear relationship between peak current against the concentration of ascorbic acid was observed within the ranges from 1 µM to 10 µM. The detection limit was measured at signal/noise (S/N) of 3. The present method can be utilized for the electrochemical detection of ascorbic acid present in food products like fruit juices.

Aniline oligomers are considered as one of the electron transfer mediators for the electrochemical oxidation of ascorbic acid. The electrochemical oxidation of ascorbic acid was investigated using aniline oligomer-functionalized polymer modified electrode. In the present investigation, we demonstrated a novel methodology for the in-situ modification of aniline oligomer onto the layered graphene sheet by using diazonium salt form as precursor molecule. An enhanced electrocatalytic current was obtained for the oxidation of ascorbic acid using aniline pentamer-functionalized reduced graphene oxide (AP-rGO). Detailed studies have been carried out to study the surface modified rGO by FTIR spectroscopy. A linear relationship between peak current against the concentration of ascorbic acid was observed within the ranges from 1 µM to 10 µM. The detection limit was measured at signal/noise (S/N) of 3. The present method can be utilized for the electrochemical detection of ascorbic acid present in food products like fruit juices.

graphene oxidepentamervoltammetric methodascorbic acidfood samplesThe authors (Dr. K. P and S. M.) are grateful to DST purse programme to upgrade the Gamry 330 electro- chemical system (DPV software).ReferencesDeNobili M.D., Perez C.D., Navarro D.A., Stortz C.A., Rojas A.M. Hydrolytic stability of L-(+)-ascorbic acid in low methoxyl pectin films with potential antioxidant activity at food interfaces. Food Bioprocess Technol., 2011, 6, P. 186–197.DeNobili M.D., Perez C.D., Navarro D.A., Stortz C.A., Rojas A.M. Hydrolytic stability of L-(+)-ascorbic acid in low methoxyl pectin films with potential antioxidant activity at food interfaces. Food Bioprocess Technol., 2011, 6, P. 186–197.Jens L. Determination of ascorbic acid and dehydroascorbic acid in biological samples by high performance liquid chromatography using subtraction methods: Reliable reduction with tris (2-carboxyethyl) phosphine hydrochloride. Anal. Biochem., 2000, 282, P. 89–93.Jens L. Determination of ascorbic acid and dehydroascorbic acid in biological samples by high performance liquid chromatography using subtraction methods: Reliable reduction with tris (2-carboxyethyl) phosphine hydrochloride. Anal. Biochem., 2000, 282, P. 89–93.Yu Y.H., Donat P.H. UV-B-induced formation of reactive oxygen species and oxidative damage of the cyanobacterium Anabaena sp.: protective effects of ascorbic acid and N-acetyl-L-cysteine. J. Photochem. Photobio. B: Biology, 2002, 66, P. 115–124.Yu Y.H., Donat P.H. UV-B-induced formation of reactive oxygen species and oxidative damage of the cyanobacterium Anabaena sp.: protective effects of ascorbic acid and N-acetyl-L-cysteine. J. Photochem. Photobio. B: Biology, 2002, 66, P. 115–124.Wu T., Guan Y., Ye J. Determination of flavonoids and ascorbic acid in grapefruit peel and juice by capillary electrophoresis with electrochemical detection. Food Chem., 2007, 100, P. 1573–1579.Wu T., Guan Y., Ye J. Determination of flavonoids and ascorbic acid in grapefruit peel and juice by capillary electrophoresis with electrochemical detection. Food Chem., 2007, 100, P. 1573–1579.Ping J., Wang Y., Wu J., Ying Y., Ji F. Determination of ascorbic acid levels in food samples by using an ionic liquid carbon nanotube composite electrode. Food Chem., 2012, 135, P. 362–367.Ping J., Wang Y., Wu J., Ying Y., Ji F. Determination of ascorbic acid levels in food samples by using an ionic liquid carbon nanotube composite electrode. Food Chem., 2012, 135, P. 362–367.Francis F.J. Wiley encyclopedia of food science and technology, 2-nd ed., Vol 4. Wiley, New York, 1999.Francis F.J. Wiley encyclopedia of food science and technology, 2-nd ed., Vol 4. Wiley, New York, 1999.Habibi B., Pournaghi-Azar M.H. Simultaneous determination of ascorbic acid, dopamine and uric acid by use of a MWCNT modified carbon-ceramic electrode and differential pulse voltammetry. Electrochimica Acta, 2012, 55, P. 5492–5498.Habibi B., Pournaghi-Azar M.H. Simultaneous determination of ascorbic acid, dopamine and uric acid by use of a MWCNT modified carbon-ceramic electrode and differential pulse voltammetry. Electrochimica Acta, 2012, 55, P. 5492–5498.Roy P.R., Saha M.S., Okajima T., Ohsaka T. Electrooxidation and amperometric detection of ascorbic acid at GC electrode modified by electropolymerization of N, N-Dimethylaniline. Electroanalysis, 2004, 16, P. 289–297.Roy P.R., Saha M.S., Okajima T., Ohsaka T. Electrooxidation and amperometric detection of ascorbic acid at GC electrode modified by electropolymerization of N, N-Dimethylaniline. Electroanalysis, 2004, 16, P. 289–297.Florou A.B., Prodromidis M.I., Stella M.T.K., Miltiades I. Fabrication and voltammetric study of lanthanum 2,6-dichlorophenolindophenol chemically modified screen printed eletrodes: Application for the determination of ascorbic acid. Anal. Chimica Acta, 2000, 423, P. 107– 114.Florou A.B., Prodromidis M.I., Stella M.T.K., Miltiades I. Fabrication and voltammetric study of lanthanum 2,6-dichlorophenolindophenol chemically modified screen printed eletrodes: Application for the determination of ascorbic acid. Anal. Chimica Acta, 2000, 423, P. 107– 114.Zuo X., Zhang H., Li N. An electrochemical biosensor for determination of ascorbic acid by cobalt (II) phthalocyanine- multiwalled carbon nanotubes modified glassy carbon electrode. Sensors Actuat., 2012, 161, P. 1074–1079.Zuo X., Zhang H., Li N. An electrochemical biosensor for determination of ascorbic acid by cobalt (II) phthalocyanine- multiwalled carbon nanotubes modified glassy carbon electrode. Sensors Actuat., 2012, 161, P. 1074–1079.Hummers W.S., Offemn R.E. Preparation of graphitic oxide. J. Am. Chem. Soc., 1958, 80, P. 1339.Hummers W.S., Offemn R.E. Preparation of graphitic oxide. J. Am. Chem. Soc., 1958, 80, P. 1339.Huang L., Hu J., Lang L., Wang X., Zhang P., Jing X., Wang X., Chen X., Lelkes P.I., MacDiarmid A.G., Wei Y. Synthesis and characterization of electroactive and biodegradable ABA block copolymer of polylactide and aniline pentamer. Biomaterials, 2007, 28, P. 1741–1751.Huang L., Hu J., Lang L., Wang X., Zhang P., Jing X., Wang X., Chen X., Lelkes P.I., MacDiarmid A.G., Wei Y. Synthesis and characterization of electroactive and biodegradable ABA block copolymer of polylactide and aniline pentamer. Biomaterials, 2007, 28, P. 1741–1751.

The authors declare that there are no conflicts of interest present.