najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМО10.17586/2220-8054-2018-9-1-120-122najo-1415Research ArticleСтатьиNew superparamagnetic fluorescent Fe@C-C5ON2H10-Alexa Fluor 647 nanoparticles for biological applicationsGaraninaA. S.KireevI. I.AlievaI. B.MajougaA. G.DavydovV. A.MurugesanS.KhabasheskuV. N.AgafonovV. N.UzbekovR. E.Lomonosov Moscow State UniversityRussian Federation20182408202591120–122Copyright © Garanina A.S., Kireev I.I., Alieva I.B., Majouga A.G., Davydov V.A., Murugesan S., Khabashesku V.N., Agafonov V.N., Uzbekov R.E., 20252025Garanina A.S., Kireev I.I., Alieva I.B., Majouga A.G., Davydov V.A., Murugesan S., Khabashesku V.N., Agafonov V.N., Uzbekov R.E.Garanina A.S., Kireev I.I., Alieva I.B., Majouga A.G., Davydov V.A., Murugesan S., Khabashesku V.N., Agafonov V.N., Uzbekov R.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/1415

The structure and physical properties of superparamagnetic Fe@C nanoparticles (Fe@C NPs) as well as their uptake by living cells and behavior inside the cell were investigated. Magnetic capacity of Fe@C NPs was compared with Fe7C3@C NPs investigated in our previous work, and showed higher value of magnetic saturation, 75 emu.g−1 (75 Am2·kg−1), against 54 emu.g−1 (54 Am2·kg−1) for Fe7C3@C. The surface of Fe@C NPs was alkylcarboxylated and further aminated for covalent linking to the molecules of fluorochrome Alexa Fluor 647. Fluorescent Fe@C-C5ON2H10-Alexa Fluor 647 NPs (Fe@C-Alexa NPs) were incubated with HT1080 human fibrosarcoma cells and investigated using fluorescent, confocal laser scanning and transmission electron microscopy. No toxic effect on the cell physiology was observed. In a magnetic field, the NPs became aligned along the magnetic lines inside the cells.

 

superparamagnetic fluorescent Fe@C nanoparticleselectron microscopymagnetic fieldThis study was supported by the Russian Foundation for Basic Research (grants 18-03-00936, 15-04-08550), Program of MSU development (PNR 5.13), Russian Science Foundation (17-15-01290) and IBiSA Electron Mi- croscopy Facility of Francois Rabelais University.ReferencesUzbekov R.E., Kireev I.I., Alieva I.B., Davydov V.A., Rakhmanina A.V., Agafonov V. Interaction of Iron Carbide Nanoparticles pro- tected by Carbon Shell Onion-like Structure with living Cells. Joint Intern. Conf. “Advanced Carbon Nanostructures” (ACNS’ 2013), St.Petersburg, Russia, P. 60.Uzbekov R.E., Kireev I.I., Alieva I.B., Davydov V.A., Rakhmanina A.V., Agafonov V. Interaction of Iron Carbide Nanoparticles pro- tected by Carbon Shell Onion-like Structure with living Cells. Joint Intern. Conf. “Advanced Carbon Nanostructures” (ACNS’ 2013), St.Petersburg, Russia, P. 60.Davydov V., Rakhmanina A., Kireev I., Alieva I., Zhironkina O., Strelkova O., Dianova V., Samani T. D., Mireles K., Yahia L.H., Uzbekov R., Agafonov V., Khabashesku V. Solid State Synthesis of Carbon-Encapsulated Iron Carbide Nanoparticles and Their Interaction with Living Cells. J. Mater. Chem. B, 2014, 2, P. 4250–4261.Davydov V., Rakhmanina A., Kireev I., Alieva I., Zhironkina O., Strelkova O., Dianova V., Samani T. D., Mireles K., Yahia L.H., Uzbekov R., Agafonov V., Khabashesku V. Solid State Synthesis of Carbon-Encapsulated Iron Carbide Nanoparticles and Their Interaction with Living Cells. J. Mater. Chem. B, 2014, 2, P. 4250–4261.Alieva I., Kireev I., Rakhmanina A., Garanina A., Strelkova O., Zhironkina O., Cherepaninets V., Davydov V., Khabashesku V., Agafonov V., Uzbekov R. Magnet-induced behavior of Iron Carbide (Fe7C3@C) Nanoparticles in the Cytoplasm of Living Cells. Nanosystems: Phys., Chem., Math., 2016, 7(1), P. 158–160.Alieva I., Kireev I., Rakhmanina A., Garanina A., Strelkova O., Zhironkina O., Cherepaninets V., Davydov V., Khabashesku V., Agafonov V., Uzbekov R. Magnet-induced behavior of Iron Carbide (Fe7C3@C) Nanoparticles in the Cytoplasm of Living Cells. Nanosystems: Phys., Chem., Math., 2016, 7(1), P. 158–160.Alieva I., Kireev I., Garanina A., Alyabyeva N., Ruyter A., Strelkova O., Zhironkina O., Cherepaninets V., Majouga A.G., Davydov V., Khabashesku V., Agafonov V., Uzbekov R. Magnetocontrollability of Fe7C3@C superparamagnetic nanoparticles in living cells. J. Nanobiotechnology, 2016, 14(1), P. 67–78.Alieva I., Kireev I., Garanina A., Alyabyeva N., Ruyter A., Strelkova O., Zhironkina O., Cherepaninets V., Majouga A.G., Davydov V., Khabashesku V., Agafonov V., Uzbekov R. Magnetocontrollability of Fe7C3@C superparamagnetic nanoparticles in living cells. J. Nanobiotechnology, 2016, 14(1), P. 67–78.Peng H., Alemany L., Margrave J., Khabashesku V. Sidewall Carboxylic Acid Functionalization of Single-Walled Carbon Nanotubes. J. Am. Chem. Soc., 2003, 125, P. 15174–15182.Peng H., Alemany L., Margrave J., Khabashesku V. Sidewall Carboxylic Acid Functionalization of Single-Walled Carbon Nanotubes. J. Am. Chem. Soc., 2003, 125, P. 15174–15182.

The authors declare that there are no conflicts of interest present.