najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМО10.17586/2220-8054-2023-14-2-223-230najo-147Research ArticleCHEMISTRY AND MATERIALS SCIENCEХИМИЯ И НАУКА О МАТЕРИАЛАХCeric phosphates and nanocrystalline ceria: selective toxicity to melanoma cellsОртофосфаты церия(IV) и нанокристаллический диоксид церия: селективная токсичность к клеткам меланомыhttps://orcid.org/0000-0002-9757-9148KozlovaT. O.

Taisiya O. Kozlova

taisia.shekunova@yandex.ruhttps://orcid.org/0000-0003-2643-4846PopovA. L.

Anton L. Popov

Pushchino

antonpopovleonid@gmail.comRomanovM. V.

Mikhail V. Romanov

Pushchino

rmvya@yandex.ruSavintsevaI. V.

Irina V. Savintseva

Pushchino

savintsevairina@mail.ruVasilyevaD. N.

Darya N. Vasilyeva

Moscow

dnvasileva1@edu.hse.ruhttps://orcid.org/0000-0002-2378-7446BaranchikovA. E.

Alexander E. Baranchikov

Moscow

a.baranchikov@yandex.ruhttps://orcid.org/0000-0003-2343-2140IvanovV. K.

Vladimir K. Ivanov

Moscow

van@igic.ras.ruKurnakov Institute of General and Inorganic Chemistry of the Russian Academy of SciencesRussian FederationInstitute of Theoretical and Experimental Biophysics of the Russian Academy of SciencesRussian FederationInstitute of Theoretical and Experimental Biophysics of the Russian AcademyofSciencesRussian FederationKurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences; National Research University Higher School of EconomicsRussian Federation202303062025142223230Copyright © Kozlova T.O., Popov A.L., Romanov M.V., Savintseva I.V., Vasilyeva D.N., Baranchikov A.E., Ivanov V.K., 20252025Kozlova T.O., Popov A.L., Romanov M.V., Savintseva I.V., Vasilyeva D.N., Baranchikov A.E., Ivanov V.K.Kozlova T.O., Popov A.L., Romanov M.V., Savintseva I.V., Vasilyeva D.N., Baranchikov A.E., Ivanov V.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/147

Nanocrystalline cerium dioxide is a promising inorganic UV filter for sunscreen applications due to its high UV absorbance and non-toxicity to normal cells. Nanoscale CeO2 also showed selective cytotoxi city to cancer cells, thus ceria-containing materials are now regarded for the creation of both preventive and therapeutic compositions. At the same time, the interaction of ceria nanoparticles with cell membranes and phosphate-rich components of sunscreen compositions arise the interest to biocompatibility of ceric phos phates. Crystalline cerium(IV) phosphates can be a promising alternative for nanoscale CeO2 due to their low solubility, high redox stability and UV protective property. However, to date, there is no information on their toxicity to cancer cells. In this work, using the MTT, Live/Dead and MMP assays, we demonstrated for the first time that the inhibitory impact of ceric phosphates Ce(PO4)(HPO4)0.5(H2O)0.5 and NH4Ce2(PO4)3 on murine melanoma B16/F10 cell line in vitro is comparable to that of nanoscale CeO2, at high (500–1000 g/ml) concentrations.

Нанокристаллический диоксид церия является многообещающим неорганическим УФ-фильтром в составе солнцезащитных средств благодаря высокому коэффициенту поглощению УФ-излучения и отсутствию токсичности по отношению к нормальным клеткам. Кроме того, наноразмерный CeO2 демонстрирует селективную цитотоксичность по отношению к раковым клеткам, поэтому церийсодержащие материалы в настоящее время рассматриваются для создания профилактических и лечебных композиций. Взаимодействие наночастиц диоксида церия с клеточными мембранами и с фосфат-содержащими компонентами солнцезащитных композиций вызывает очевидный интерес к биосовместимости фосфатов церия(IV). Кристаллические ортофосфаты церия(IV) могут стать многообещающей альтернативой наноразмерному CeO2 благодаря их низкой растворимости, высокой редокс-стабильности и УФ-защитным свойствам. Однако на сегодняшний день нет информации об их токсичности по отношению к раковым клеткам. В данной работе с использованием тестов MTT, Live/Dead и MMP мы впервые продемонстрировали ингибирующее влияние ортофосфатов церия(IV) Ce(PO4)(HPO4)0.5(H2O)0.5 и NH4Ce2(PO4)3 на клеточную линию меланомы мыши B16/F10 in vitro, сопоставимое с действием наноразмерного CeO2, при высоких (500–1000 мкг/мл) концентрациях. 

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The authors declare that there are no conflicts of interest present.