najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМОnajo-1520Research ArticleCHEMISTRY AND MATERIALS SCIENCEХИМИЯ И НАУКА О МАТЕРИАЛАХUV-tuning the redox properties of nanoscale cerium dioxide and its enzyme conjugateshttps://orcid.org/0000-0002-5868-4746SozarukovaMadina M.

Senior Researcher, 9268-1063

s_madinam@bk.ruhttps://orcid.org/0000-0002-2725-8891FilippovaArina D.arifilippova@yandex.ruhttps://orcid.org/0009-0006-0606-8059RatovaDaria-Maria V.

Junior researcher, 2467-1574

darmarrat@gmail.comhttps://orcid.org/0000-0002-2383-1697MikheevIvan V.

Associate Professor, 7938-0617

mikheev.ivan@gmail.comhttps://orcid.org/0000-0002-8243-6339ProskurninaElena V.

Chief Researcher, 8072-7745

proskurnina@gmail.comhttps://orcid.org/0000-0002-2378-7446BaranchikovAlexander E.

Leading Researcher, Head of the Laboratory, 6648-2744

a.baranchikov@yandex.ruhttps://orcid.org/0000-0003-2343-2140IvanovVladimir K.

Director, 8732-4200

van@igic.ras.ruKurnakov Institute of General and Inorganic Chemistry of the Russian Academy of SciencesRussian FederationAnalytical Chemistry Division, Chemistry Department, M.V. Lomonosov Moscow State UniversityRussian Federation2025050220261661520Copyright © Sozarukova M.M., Filippova A.D., Ratova D.V., Mikheev I.V., Proskurnina E.V., Baranchikov A.E., Ivanov V.K., 20262026Sozarukova M.M., Filippova A.D., Ratova D.V., Mikheev I.V., Proskurnina E.V., Baranchikov A.E., Ivanov V.K.Sozarukova M.M., Filippova A.D., Ratova D.V., Mikheev I.V., Proskurnina E.V., Baranchikov A.E., Ivanov V.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/1520

This study investigated the redox properties of cerium oxide nanoparticles (CeO2 NPs) and their conjugates with superoxide dismutase (SOD) or horseradish peroxidase (HRP) as well as the UV-induced modulation of these properties. UV exposure non-monotonically decreased the SOD-like property of the bare CeO2 NPs. The CeO2 conjugates with enzymes were analyzed both immediately after preparation and after being aged for 3 h. Chemiluminescence assays showed the synergistic effect for the CeO2-SOD conjugates which showed high SOD activity. Additionally, CeO2 NPs enhanced the stability of the conjugated SOD under UV exposure thus demonstrating a photoprotective function. The CeO2-HRP conjugates demonstrated lower prooxidant activity compared to the bare enzyme, however higher stability under UV irradiation. The effect of UV radiation on CeO2-HRP conjugates was found to be multidirectional and depended on the incubation time of the CeO2 NPs with the enzyme. The results demonstrated that CeO2-enzyme conjugates offer tunable dual functionality and UV light could be an important parameter affecting their redox properties. The latter effect should be taken into account for designing advanced cosmeceutical formulations.

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The authors declare that there are no conflicts of interest present.