najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМО10.17586/2220-8054-2023-14-6-644-651najo-154Research ArticleCHEMISTRY AND MATERIALS SCIENCEХИМИЯ И НАУКА О МАТЕРИАЛАХCerium dioxide nanoparticles modulate the oxidative metabolism of neutrophils upon blood irradiation with a pulsed broadband UV sourceНаночастицы диоксида церия модулируют оксидативный метаболизм нейтрофилов при облучении крови импульсным широкополосным источником УФ-излученияhttps://orcid.org/0000-0002-5868-4746СозаруковаМ. М.SozarukovaM. M.

Madina M. Sozarukova

Leninsky av., 31, Moscow, 119991

s_madinam@bk.ruhttps://orcid.org/0009-0005-6923-6120ЧиликинаП. А.ChilikinaP. A.

Polina A. Chilikina

Miklukho-Maklaya Str., 16/10, Moscow, 117997

p.chilikina@gmail.comhttps://orcid.org/0009-0005-5806-9603НовиковД. О.NovikovD. O.

Dmitry O. Novikov

Moscow region, Chernogolovka

zerooo@list.ruhttps://orcid.org/0000-0002-8243-6339ПроскурнинаЕ. В.ProskurninaE. V.

Elena V. Proskurnina

ul. Moskvorechye 1, Moscow, 115522

proskurnina@gmail.comhttps://orcid.org/0000-0002-2378-7446БаранчиковА. Е.BaranchikovA. E.

Alexander E. Baranchikov

Leninsky av., 31, Moscow, 119991

a.baranchikov@yandex.ruhttps://orcid.org/0000-0003-2343-2140ИвановВ. К.IvanovV. K.

Vladimir K. Ivanov

Leninsky av., 31, Moscow, 119991

van@igic.ras.ruKurnakov Institute of General and Inorganic Chemistry of the Russian Academy of SciencesRussian FederationScientific and Industrial Enterprise “MELITTA” LtdRussian FederationJoint Stock Company “Experimental Factory for Scientific Engineering of the Russian Academy of Sciences”Russian FederationResearch Centre for Medical GeneticsRussian Federation202302062025146644651Copyright © Sozarukova M.M., Chilikina P.A., Novikov D.O., Proskurnina E.V., Baranchikov A.E., Ivanov V.K., 20252025Созарукова М.М., Чиликина П.А., Новиков Д.О., Проскурнина Е.В., Баранчиков А.Е., Иванов В.К.Sozarukova M.M., Chilikina P.A., Novikov D.O., Proskurnina E.V., Baranchikov A.E., Ivanov V.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/154

The effect of CeO2 nanoparticles on the oxidative activity of neutrophils under UV irradiation with a pulsed broadband UV source (210 mJ/cm2) was analyzed. The effects of citrate-stabilized CeO2 sol on spontaneous and stimulated by phorbol-12-myristate-13-acetate (PMA) and N-formylmethionyl-leucyl-phenylalanine (fMLP) luminol-dependent chemiluminescence of neutrophils were evaluated. The activating effect of CeO2 nanoparticles on the spontaneous and suppressive effect on the stimulated chemiluminescence of blood neutrophils from apparently healthy donors was shown, with the most pronounced activating effect of CeO2 nanoparticles revealed in the blood sample with initially high radical-producing cell activity. Under UV irradiation of blood at a dose of 210 mJ/cm2 CeO2 nanoparticles enhance both spontaneous and stimulated radical-producing function of neutrophils. Probably, the suppressive and activating effects of citrate-stabilised cerium dioxide sol may be due to the antioxidant activity of CeO2 nanoparticles with respect to hypochlorite ions and prooxidant activity with respect to hydrogen peroxide.

Проведен анализ влияния наночастиц СеО2 на оксидативную активность нейтрофилов при облучении импульсным широкополосным источником УФ-излучения (210 мДж/см2). Оценены эффекты цитрат-стабилизированного золя СеО2 на спонтанную и стимулированную с помощью форбол-12-миристат-13-ацетата (ФМА) и N-формилметионил-лейцил-фенилаланина (фМЛФ) люминол-зависимую хемилюминесценцию нейтрофилов. Показано активирующее влияние наночастиц СеО2 на спонтанную хемилюминесценцию и супрессорное действие на стимулированную хемилюминесценцию нейтрофилов крови практически здоровых доноров, при этом наиболее выраженный активирующий эффект нанодисперсный СеО2 проявляет в отношении образца крови с изначально высокой радикал-продуцирующей активностью клеток. При УФ-облучении крови в дозе 210 мДж/см2 наночастицы СеО2 усиливают как спонтанную, так и стимулированную радикал-продуцирующую функцию нейтрофилов. Супрессорное и активирующее действие цитратного золя диоксида церия может быть обусловлено антиоксидантной активностью наночастиц СеО2 по отношению к гипохлорит-ионам и прооксидантной активностью по отношению к пероксиду водорода, соответственно

нанозимынаночастицы диоксида цериянейтрофилыУФ-излучениеимпульсная ксеноновая УФ-лампахемилюминесценцияnanozymescerium dioxide nanoparticlesneutrophilsUV radiationpulsed xenon UV lampchemiluminescenceThis work was supported by the Russian Science Foundation (project 21-73-00251).ReferencesSingh S. Cerium oxide based nanozymes: Redox phenomenon at biointerfaces. Biointerphases, 2016, 11 (4), 04B202.Singh S. Cerium oxide based nanozymes: Redox phenomenon at biointerfaces. Biointerphases, 2016, 11 (4), 04B202.Jiang D., Ni D., et al. Nanozyme: new horizons for responsive biomedical applications. Chem. Soc. Rev., 2019, 48 (14), P. 3683–3704.Jiang D., Ni D., et al. Nanozyme: new horizons for responsive biomedical applications. Chem. Soc. Rev., 2019, 48 (14), P. 3683–3704.Singh K.R., Nayak V., et al. Cerium oxide nanoparticles: properties, biosynthesis and biomedical application. RSC Adv., 2020, 10 (45), P. 27194–27214.Singh K.R., Nayak V., et al. Cerium oxide nanoparticles: properties, biosynthesis and biomedical application. RSC Adv., 2020, 10 (45), P. 27194–27214.Filippova A.D., Sozarukova M.M., et al. Peroxidase-like activity of CeO2 nanozymes: particle size and chemical environment matter. Molecules, 2023, 28 (9), 3811.Filippova A.D., Sozarukova M.M., et al. Peroxidase-like activity of CeO2 nanozymes: particle size and chemical environment matter. Molecules, 2023, 28 (9), 3811.Ivanov V.K., Polezhaeva O.S., Tret’yakov Y.D. Nanocrystalline ceria: synthesis, structure-sensitive properties, and promising applications. Russ. J. Gen. Chem., 2010, 80 (3), P. 604–617.Ivanov V.K., Polezhaeva O.S., Tret’yakov Y.D. Nanocrystalline ceria: synthesis, structure-sensitive properties, and promising applications. Russ. J. Gen. Chem., 2010, 80 (3), P. 604–617.Shcherbakov A.B., Reukov V.V., et. al. CeO2 nanoparticle-containing polymers for biomedical applications: A review. Polymers, 2021, 13 (6), 924.Shcherbakov A.B., Reukov V.V., et. al. CeO2 nanoparticle-containing polymers for biomedical applications: A review. Polymers, 2021, 13 (6), 924.Popov A.L., Shcherbakov A.B., et. al. Cerium dioxide nanoparticles as third-generation enzymes (nanozymes). Nanosyst: Phys, Chem, Math., 2017, 8 (6), P. 760–781.Popov A.L., Shcherbakov A.B., et. al. Cerium dioxide nanoparticles as third-generation enzymes (nanozymes). Nanosyst: Phys, Chem, Math., 2017, 8 (6), P. 760–781.Korsvik C., Patil S., et al. Superoxide dismutase mimetic properties exhibited by vacancy engineered ceria nanoparticles. Chem. Commun. (Camb.), 2007, 10, P. 1056–1058.Korsvik C., Patil S., et al. Superoxide dismutase mimetic properties exhibited by vacancy engineered ceria nanoparticles. Chem. Commun. (Camb.), 2007, 10, P. 1056–1058.Heckert E.G., Karakoti A., et al. The role of cerium redox state in the SOD mimetic activity of nanoceria. Biomaterials, 2008, 29 (18), P. 2705– 2709.Heckert E.G., Karakoti A., et al. The role of cerium redox state in the SOD mimetic activity of nanoceria. Biomaterials, 2008, 29 (18), P. 2705– 2709.Baldim V., Bedioui F., et al. The enzyme-like catalytic activity of cerium oxide nanoparticles and its dependency on Ce3+ surface area concentration. Nanoscale, 2018, 10 (15), P. 6971–6980.Baldim V., Bedioui F., et al. The enzyme-like catalytic activity of cerium oxide nanoparticles and its dependency on Ce3+ surface area concentration. Nanoscale, 2018, 10 (15), P. 6971–6980.Sozarukova M.M., Shestakova M.A., et al. Quantification of free radical scavenging properties and SOD-like activity of cerium dioxide nanoparticles in biochemical models. Rus. J. Inorg. Chem., 2020, 65, P. 597–605.Sozarukova M.M., Shestakova M.A., et al. Quantification of free radical scavenging properties and SOD-like activity of cerium dioxide nanoparticles in biochemical models. Rus. J. Inorg. Chem., 2020, 65, P. 597–605.Pirmohamed T., Dowding J.M., et al., Nanoceria exhibit redox state-dependent catalase mimetic activity. ChemComm., 2010, 46 (16), P. 2736– 2738.Pirmohamed T., Dowding J.M., et al., Nanoceria exhibit redox state-dependent catalase mimetic activity. ChemComm., 2010, 46 (16), P. 2736– 2738.Singh R., Singh S. Role of phosphate on stability and catalase mimetic activity of cerium oxide nanoparticles. Colloids Surf. B Biointerfaces, 2015, 132, P. 78–84.Singh R., Singh S. Role of phosphate on stability and catalase mimetic activity of cerium oxide nanoparticles. Colloids Surf. B Biointerfaces, 2015, 132, P. 78–84.Singh R., Singh S. Redox-dependent catalase mimetic cerium oxide-based nanozyme protect human hepatic cells from 3-AT induced acatalasemia. Colloids Surf. B Biointerfaces, 2019, 175, P. 625–635.Singh R., Singh S. Redox-dependent catalase mimetic cerium oxide-based nanozyme protect human hepatic cells from 3-AT induced acatalasemia. Colloids Surf. B Biointerfaces, 2019, 175, P. 625–635.Jiao X., Song H., et al., Well-redispersed ceria nanoparticles: promising peroxidase mimetics for H2O2 and glucose detection. Anal. Methods, 2012, 4 (10), P. 3261–3267.Jiao X., Song H., et al., Well-redispersed ceria nanoparticles: promising peroxidase mimetics for H2O2 and glucose detection. Anal. Methods, 2012, 4 (10), P. 3261–3267.Ansari A.A., Solanki P.R., Malhotra B. Hydrogen peroxide sensor based on horseradish peroxidase immobilized nanostructured cerium oxide film. J. Biotechnol., 2009, 142 (2), P. 179–184.Ansari A.A., Solanki P.R., Malhotra B. Hydrogen peroxide sensor based on horseradish peroxidase immobilized nanostructured cerium oxide film. J. Biotechnol., 2009, 142 (2), P. 179–184.Sozarukova M.M., Proskurnina E.V., Ivanov V.K. Prooxidant potential of CeO2 nanoparticles towards hydrogen peroxide. Nanosyst: Phys, Chem, Math., 2021, 12 (3), P. 283–290.Sozarukova M.M., Proskurnina E.V., Ivanov V.K. Prooxidant potential of CeO2 nanoparticles towards hydrogen peroxide. Nanosyst: Phys, Chem, Math., 2021, 12 (3), P. 283–290.Filippova A.D, Sozarukova M.M., et al. Low-Temperature Inactivation of Enzyme-like Activity of Nanocrystalline CeO2 Sols. Rus. J. Inorg. Chem., 2022, 67 (12), P. 1948–1955.Filippova A.D, Sozarukova M.M., et al. Low-Temperature Inactivation of Enzyme-like Activity of Nanocrystalline CeO2 Sols. Rus. J. Inorg. Chem., 2022, 67 (12), P. 1948–1955.Liu H., Liu J. Self-limited Phosphatase-mimicking CeO2 Nanozymes. Chem.Nano.Mat, 2020, 6 (6), P. 947–952.Liu H., Liu J. Self-limited Phosphatase-mimicking CeO2 Nanozymes. Chem.Nano.Mat, 2020, 6 (6), P. 947–952.Khulbe K., Karmakar K., et al. Nanoceria-based phospholipase-mimetic cell membrane disruptive antibiofilm agents. ACS Appl. Bio Mater., 2020, 3 (7), P. 4316–4328.Khulbe K., Karmakar K., et al. Nanoceria-based phospholipase-mimetic cell membrane disruptive antibiofilm agents. ACS Appl. Bio Mater., 2020, 3 (7), P. 4316–4328.Tian Z., Yao T., et al. Photolyase-like catalytic behavior of CeO2. Nano Lett., 2019, 19 (11), P. 8270–8277.Tian Z., Yao T., et al. Photolyase-like catalytic behavior of CeO2. Nano Lett., 2019, 19 (11), P. 8270–8277.Liu D., Yang P., et al. Study on performance of mimic uricase and its application in enzyme-free analysis. Anal. Bioanal. Chem., 2021, 413 (26), P. 6571–6580.Liu D., Yang P., et al. Study on performance of mimic uricase and its application in enzyme-free analysis. Anal. Bioanal. Chem., 2021, 413 (26), P. 6571–6580.Celardo I., Pedersen J.Z., et al. Pharmacological potential of cerium oxide nanoparticles. Nanoscale, 2011, 3 (4), P. 1411–1420.Celardo I., Pedersen J.Z., et al. Pharmacological potential of cerium oxide nanoparticles. Nanoscale, 2011, 3 (4), P. 1411–1420.Ciccarese F., Raimondi V., et al. Nanoparticles as tools to target redox homeostasis in cancer cells. Antioxidants, 2020, 9 (3), 211.Ciccarese F., Raimondi V., et al. Nanoparticles as tools to target redox homeostasis in cancer cells. Antioxidants, 2020, 9 (3), 211.Sun W., Yang Y. Recent advances in redox-responsive nanoparticles for combined cancer therapy. Nanoscale Adv., 2022, 4 (17), P. 3504–3516.Sun W., Yang Y. Recent advances in redox-responsive nanoparticles for combined cancer therapy. Nanoscale Adv., 2022, 4 (17), P. 3504–3516.Hamblin M.R. Ultraviolet irradiation of blood: “the cure that time forgot”? Adv. Exp. Med. Biol., 2017, 996, P. 295–309.Hamblin M.R. Ultraviolet irradiation of blood: “the cure that time forgot”? Adv. Exp. Med. Biol., 2017, 996, P. 295–309.Ahmad S.I. Ultraviolet light in human health, diseases and environment. Springer International Publishing, Cham, 2017, 365 p.Ahmad S.I. Ultraviolet light in human health, diseases and environment. Springer International Publishing, Cham, 2017, 365 p.Heilingloh C.S., Aufderhorst U.W., et al. Susceptibility of SARS-CoV-2 to UV irradiation. Am. J. Infect. Control, 2020. 48 (10), P. 1273–1275.Heilingloh C.S., Aufderhorst U.W., et al. Susceptibility of SARS-CoV-2 to UV irradiation. Am. J. Infect. Control, 2020. 48 (10), P. 1273–1275.Hessling M., H¨ones K., et al. Ultraviolet irradiation doses for coronavirus inactivation–review and analysis of coronavirus photoinactivation studies. GMS Hyg. Infect. Control, 2020, 15.Hessling M., H¨ones K., et al. Ultraviolet irradiation doses for coronavirus inactivation–review and analysis of coronavirus photoinactivation studies. GMS Hyg. Infect. Control, 2020, 15.Boretti A., Banik B., Castelletto S. Use of ultraviolet blood irradiation against viral infections. Clin. Rev. Allergy Immunol., 2021, 60, P. 259–270.Boretti A., Banik B., Castelletto S. Use of ultraviolet blood irradiation against viral infections. Clin. Rev. Allergy Immunol., 2021, 60, P. 259–270.Peloi K.E., Ratti B.A., et al. Engineered nanoceria modulate neutrophil oxidative response to low doses of UV-B radiation through the inhibition of reactive oxygen species production. J. Biomed. Mater. Res. A, 2021, 109 (12), P. 2570–2579.Peloi K.E., Ratti B.A., et al. Engineered nanoceria modulate neutrophil oxidative response to low doses of UV-B radiation through the inhibition of reactive oxygen species production. J. Biomed. Mater. Res. A, 2021, 109 (12), P. 2570–2579.Lin M.-H., Lin C.-F., et al. The interplay between nanoparticles and neutrophils. J. Biomed. Nanotechnol., 2018, 14 (1), P. 66–85.Lin M.-H., Lin C.-F., et al. The interplay between nanoparticles and neutrophils. J. Biomed. Nanotechnol., 2018, 14 (1), P. 66–85.Nauseef W.M., Borregaard N. Neutrophils at work. Nat. Immunol., 2014, 15 (7), P. 602–611.Nauseef W.M., Borregaard N. Neutrophils at work. Nat. Immunol., 2014, 15 (7), P. 602–611.Mayadas T.N., Cullere X., Lowell C.A. The multifaceted functions of neutrophils. Annu. Rev. Pathol., 2014, 9, P. 181–218.Mayadas T.N., Cullere X., Lowell C.A. The multifaceted functions of neutrophils. Annu. Rev. Pathol., 2014, 9, P. 181–218.Knaapen A.M., G¨ung¨or N., et al. Neutrophils and respiratory tract DNA damage and mutagenesis: a review. Mutagenesis, 2006, 21 (4), P. 225–236.Knaapen A.M., G¨ung¨or N., et al. Neutrophils and respiratory tract DNA damage and mutagenesis: a review. Mutagenesis, 2006, 21 (4), P. 225–236.Deniset J.F., Kubes P. Recent advances in understanding neutrophils. F1000Res., 2016, 5, 2912.Deniset J.F., Kubes P. Recent advances in understanding neutrophils. F1000Res., 2016, 5, 2912.Boni B.O.O., Lamboni L. Immunomodulation and cellular response to biomaterials: the overriding role of neutrophils in healing. Mater. Horiz., 2019, 6 (6), P. 1122–1137.Boni B.O.O., Lamboni L. Immunomodulation and cellular response to biomaterials: the overriding role of neutrophils in healing. Mater. Horiz., 2019, 6 (6), P. 1122–1137.Nguyen G.T., Green E.R., Mecsas J. Neutrophils to the ROScue: mechanisms of NADPH oxidase activation and bacterial resistance. Front. Cell. Infect. Microbiol., 2017, 7, 373.Nguyen G.T., Green E.R., Mecsas J. Neutrophils to the ROScue: mechanisms of NADPH oxidase activation and bacterial resistance. Front. Cell. Infect. Microbiol., 2017, 7, 373.Chakraborty A., Boer J.C., et al. Amino acid functionalized inorganic nanoparticles as cutting-edge therapeutic and diagnostic agents. Bioconjug. Chem., 2017, 29 (3), P. 657–671.Chakraborty A., Boer J.C., et al. Amino acid functionalized inorganic nanoparticles as cutting-edge therapeutic and diagnostic agents. Bioconjug. Chem., 2017, 29 (3), P. 657–671.Sur S., Rathore A., et al. Recent developments in functionalized polymer nanoparticles for efficient drug delivery system. Nano-Struct. Nano- Objects, 2019, 20, 100397.Sur S., Rathore A., et al. Recent developments in functionalized polymer nanoparticles for efficient drug delivery system. Nano-Struct. Nano- Objects, 2019, 20, 100397.Zhang W., Bai M., et al. Safety and efficacy of regional citrate anticoagulation for continuous renal replacement therapy in liver failure patients: a systematic review and meta-analysis. Crit. Care, 2019, 23 (1), P. 1–11.Zhang W., Bai M., et al. Safety and efficacy of regional citrate anticoagulation for continuous renal replacement therapy in liver failure patients: a systematic review and meta-analysis. Crit. Care, 2019, 23 (1), P. 1–11.Shcherbakov A.B., Teplonogova M.A. et al. Facile method for fabrication of surfactant-free concentrated CeO2 sols. Mater. Res. Express, 2017, 4 (5), 055008.Shcherbakov A.B., Teplonogova M.A. et al. Facile method for fabrication of surfactant-free concentrated CeO2 sols. Mater. Res. Express, 2017, 4 (5), 055008.Obraztsov I.V., Godkov M.A., et al. An evaluation of neutrophil function in whole blood samples with two-step stimulation: a new approach to chemiluminescence analysis. Rus. J. Immun., 2015, 9, P. 418–425.Obraztsov I.V., Godkov M.A., et al. An evaluation of neutrophil function in whole blood samples with two-step stimulation: a new approach to chemiluminescence analysis. Rus. J. Immun., 2015, 9, P. 418–425.Allen R.C. Role of oxygen in phagocyte microbicidal action. Environ. Health Perspect., 1994, 102 (Suppl 10), P. 201–208.Allen R.C. Role of oxygen in phagocyte microbicidal action. Environ. Health Perspect., 1994, 102 (Suppl 10), P. 201–208.Tarafdar A., Pula G. The role of NADPH oxidases and oxidative stress in neurodegenerative disorders. Int. J. Mol. Sci., 2018, 19 (12), 3824.Tarafdar A., Pula G. The role of NADPH oxidases and oxidative stress in neurodegenerative disorders. Int. J. Mol. Sci., 2018, 19 (12), 3824.Darroudi M., Sarani, M. et al. Nanoceria: Gum mediated synthesis and in vitro viability assay. Ceram. Int., 2014, 40 (2), P. 2863–2868.Darroudi M., Sarani, M. et al. Nanoceria: Gum mediated synthesis and in vitro viability assay. Ceram. Int., 2014, 40 (2), P. 2863–2868.Li C., Zhao W., et al. Cytotoxicity of ultrafine monodispersed nanoceria on human gastric cancer cells. J. Biomed. Nanotechnol., 2014, 10 (7), P. 1231–1241.Li C., Zhao W., et al. Cytotoxicity of ultrafine monodispersed nanoceria on human gastric cancer cells. J. Biomed. Nanotechnol., 2014, 10 (7), P. 1231–1241.Shokrzadeh M., Abdi H., et al. Nanoceria attenuated high glucose-induced oxidative damage in HepG2 cells. Cell J., 2016, 18 (1), 97.Shokrzadeh M., Abdi H., et al. Nanoceria attenuated high glucose-induced oxidative damage in HepG2 cells. Cell J., 2016, 18 (1), 97.Ivanov V.K., Usatenko A.V., Shcherbakov A.B. Antioxidant activity of nanocrystalline ceria to anthocyanins. Russ. J. Inorg. Chem., 2009, 54, 1522.Ivanov V.K., Usatenko A.V., Shcherbakov A.B. Antioxidant activity of nanocrystalline ceria to anthocyanins. Russ. J. Inorg. Chem., 2009, 54, 1522.Ivanov V.K., Shcherbakov A.B., et al. Inactivation of the nitroxyl radical by ceria nanoparticles. Dokl. Chem., 2010, 430, P. 43–46.Ivanov V.K., Shcherbakov A.B., et al. Inactivation of the nitroxyl radical by ceria nanoparticles. Dokl. Chem., 2010, 430, P. 43–46.Zholobak N.M., Shcherbakov A.B., et al. Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells. RSC Adv., 2014, 4, P. 51703–51710.Zholobak N.M., Shcherbakov A.B., et al. Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells. RSC Adv., 2014, 4, P. 51703–51710.Eriksson P., Tal A.A., et al. Cerium oxide nanoparticles with antioxidant capabilities and gadolinium integration for MRI contrast enhancement. Sci. Rep., 2018, 8 (1), P. 6999.Eriksson P., Tal A.A., et al. Cerium oxide nanoparticles with antioxidant capabilities and gadolinium integration for MRI contrast enhancement. Sci. Rep., 2018, 8 (1), P. 6999.Pulido-Reyes G., Das S., et al. Hypochlorite scavenging activity of cerium oxide nanoparticles. RSC Adv., 2016, 6 (67), P. 62911–62915.Pulido-Reyes G., Das S., et al. Hypochlorite scavenging activity of cerium oxide nanoparticles. RSC Adv., 2016, 6 (67), P. 62911–62915.Brestel E.P. Co-oxidation of luminol by hypochlorite and hydrogen peroxide implications for neutrophil chemiluminescence. Biochem. Biophys. Res. Commun., 1985, 126 (1), P. 482–488.Brestel E.P. Co-oxidation of luminol by hypochlorite and hydrogen peroxide implications for neutrophil chemiluminescence. Biochem. Biophys. Res. Commun., 1985, 126 (1), P. 482–488.Edwards, S. Luminol-and lucigenin-dependent chemiluminescence of neutrophils: role of degranulation. J. Clin. Lab. Immunol., 1987, 22 (1), P. 35–39.Edwards, S. Luminol-and lucigenin-dependent chemiluminescence of neutrophils: role of degranulation. J. Clin. Lab. Immunol., 1987, 22 (1), P. 35–39.Gorudko I., Mikhalchik E., Sokolov A., Grigorieva D., Kostevich V., Vasilyev V., Cherenkevich S., Panasenko O. The production of reactive oxygen and halogen species by neutrophils in response to monomeric forms of myeloperoxidase. Biophysics, 2017, 62, P. 919–925.Gorudko I., Mikhalchik E., Sokolov A., Grigorieva D., Kostevich V., Vasilyev V., Cherenkevich S., Panasenko O. The production of reactive oxygen and halogen species by neutrophils in response to monomeric forms of myeloperoxidase. Biophysics, 2017, 62, P. 919–925.Bedouh`ene S., Moulti-Mati F., et al. Luminol-amplified chemiluminescence detects mainly superoxide anion produced by human neutrophils. Am. J. Blood Res., 2017, 7 (4), 41.Bedouh`ene S., Moulti-Mati F., et al. Luminol-amplified chemiluminescence detects mainly superoxide anion produced by human neutrophils. Am. J. Blood Res., 2017, 7 (4), 41.Seminko V., Maksimchuk P., et al. Catalytic Decomposition of Hypochlorite Anions by Ceria Nanoparticles Visualized by Spectroscopic Techniques. J. Phys. Chem. C, 2019, 123 (33), P. 20675–20681.Seminko V., Maksimchuk P., et al. Catalytic Decomposition of Hypochlorite Anions by Ceria Nanoparticles Visualized by Spectroscopic Techniques. J. Phys. Chem. C, 2019, 123 (33), P. 20675–20681.Adebayo O.A., Akinloye O., Adaramoye O.A. Cerium oxide nanoparticles attenuate oxidative stress and inflammation in the liver of diethylnitrosamine-treated mice. Biol. Trace Elem. Res., 2020, 193, P. 214–225.Adebayo O.A., Akinloye O., Adaramoye O.A. Cerium oxide nanoparticles attenuate oxidative stress and inflammation in the liver of diethylnitrosamine-treated mice. Biol. Trace Elem. Res., 2020, 193, P. 214–225.Peloi K.E., Lancheros C.A.C., et al. Antioxidative photochemoprotector effects of cerium oxide nanoparticles on UVB irradiated fibroblast cells. Colloids Surf. B Biointerfaces, 2020, 191, P. 111013.Peloi K.E., Lancheros C.A.C., et al. Antioxidative photochemoprotector effects of cerium oxide nanoparticles on UVB irradiated fibroblast cells. Colloids Surf. B Biointerfaces, 2020, 191, P. 111013.

The authors declare that there are no conflicts of interest present.