najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМО10.17586/2220-8054-2023-14-6-652-659najo-156Research ArticleCHEMISTRY AND MATERIALS SCIENCEХИМИЯ И НАУКА О МАТЕРИАЛАХSlow zinc release from carboxymethylcellulose gels filled with humic zinc oxide nanocompositesМедленное высвобождение цинка из карбоксиметилцеллюлозных гелей, наполненных гуминовыми нанокомпозитами оксида цинкаhttps://orcid.org/0009-0009-1635-3648ЛарионовК. С.LarionovK. S.

Konstantin S. Larionov – Department of Chemistry

Leninskiye Gory 1-3, 119991 Moscow

konstantin.larionov@chemistry.msu.ruhttps://orcid.org/0000-0002-8475-8091ВоликовА. Б.VolikovA.

Alexander Volikov – Department of Chemistry

Leninskiye Gory 1-3, 119991 Moscow

ab.volikov@gmail.comhttps://orcid.org/0000-0002-8210-8263СоболевН. А.SobolevN. A.

Nikita A. Sobolev – Department of Chemistry

Leninskiye Gory 1-3, 119991 Moscow

n.a.sobolev@outlook.comhttps://orcid.org/0000-0003-0620-8016КозловД. А.KozlovD. A.

Daniil A. Kozlov

Leninsky Prospect 31, 119991 Moscow

kozlov@inorg.chem.msu.ruhttps://orcid.org/0000-0001-9084-7851ПерминоваИ. В.PerminovaI. V.

Irina V. Perminova – Department of Chemistry

Leninskiye Gory 1-3, 119991 Moscow

iperm@med.chem.msu.ruLomonosov Moscow State UniversityRussian FederationKurnakov Institute of General and Inorganic Chemistry of RASRussian Federation202302062025146652659Copyright © Larionov K.S., Volikov A., Sobolev N.A., Kozlov D.A., Perminova I.V., 20252025Ларионов К.С., Воликов А.Б., Соболев Н.А., Козлов Д.А., Перминова И.В.Larionov K.S., Volikov A., Sobolev N.A., Kozlov D.A., Perminova I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/156

The study is focused on characterization of humic zinc oxide nanocomposites and their potential application in wound healing as antibacterial agent. Zinc oxide nanoparticles were synthesized with varying concentrations of humic substances (HS) and analyzed using PXRD, TEM, SEM and UV-Vis techniques. The nanoparticle sizes based on the PXRD data decreased ranged from 50 to 15 nm along with an increase in humic ligand concentration. TEM images revealed that the star-shaped aggregates of 200–500 nm ZnO particles were formed in the absence of HS, whereas the presence of humic ligands led to shapeless smaller particles ranging from 20 to 200 nm. UV-Vis spectra showed increasing of zinc oxide band gap caused with an rise of HS concentration. The band gap of ZnO nanoparticles increased from 3.19 eV to 3.40 eV as the concentration of HS increased up to 15 g/L. The synthesized ZnO-HS nanocomposites were used for filling in the hydrogels of carboxymethylcellulose (CMC). The release studies of zinc ions from the gel into different buffers were conducted to imitate wound conditions. The measurements of Zn concentrations over time in buffer showed a gradual release over time making these gels potentially suitable for long-term wound treatment.

Цель работы - исследование нанокомпозитов оксида цинка и гуминовых веществ (ГВ), направленном на оценку возможности их применении для заживления ран в виде фармацевтически активных компонентов гидрогелей. Наночастицы оксида цинка синтезировали в присутствии различных концентраций гуминовых веществ. Анализ полученных нанокомпозитов вели с использованием методов рентгеновской дифрактометрии, ПЭМ, СЭМ и УФ-видимой спектроскопии. Найдено, что размер наночастиц ZnO  уменьшался от 50 до 15 нм по мере увеличения концентрации ГВ. ПЭМ-изображения показали присутствие звездообразных агрегатов частиц ZnO размером 200-500 нм, которые формировались в отсутствие гуминовых веществ (ГВ). В присутствии ГВ наблюдалось образование бесформенных частиц с размерами в диапазоне от 20 до 200 нм. Спектры поглощения показали характерный пик ZnO около 360 нм, который смещался наряду с увеличением концентрации ГВ. Согласно выполненным расчетам, ширина запрещенной зоны наночастиц ZnO увеличилась с 3,35 эВ до 3,51 эВ по мере увеличения концентрации ГВ до 15 г/л. Это может указывать на образование дефектов в кристаллах ZnO в результате взаимодействия с ГВ. Синтезированные нанокомпозиты ZnO-HS были использованы для заполнения гидрогелей карбоксиметилцеллюлозы (КМЦ). Для имитации состояния раны были проведены исследования высвобождения ионов цинка из геля в бульон Мюллера-Хинтона и 0.1М ацетатный буфер. Динамика концентрации Zn в приемных растворах показала замедленное высвобождение с течением времени, что делает эти гели потенциально пригодными для длительного лечения ран.

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The authors declare that there are no conflicts of interest present.