References

najo

Nanosystems: Physics, Chemistry, Mathematics

Наносистемы: физика, химия, математика

2220-80542305-7971

Университет ИТМО

10.17586/2220-8054-2022-13-1-96-103

najo-221

Research Article

Статьи

Synthesis and biocompatibility study of ceria-mildronate nanocomposite in vitro

Popov

A. L.

antonpopovleonid@gmail.com

Kolmanovich

D. D.

kdd100996@mail.ru

Popova

N. R.

nellipopovaran@gmail.com

Sorokina

S. S.

sorokinasvetlana.iteb@gmail.com

Ivanova

O. S.

runetta05@mail.ru

Chukavin

N. N.

chukavinnik@gmail.com

Shcherbakov

A. B.

ceroform@gmail.com

Kozlova

T. O.

tasiok@mail.ru

Kalashnikova

S. A.

kalashnikova.sana@mail.ru

Ivanov

V. K.

van@igic.ras.ru

Institute of Theoretical and Experimental Biophysics of the Russian Academy of SciencesRussian FederationKurnakov Institute of General and Inorganic Chemistry of the Russian Academy of SciencesRussian FederationInstitute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences; Moscow Region State UniversityRussian FederationZabolotny Institute of Microbiology and VirologyRussian Federation40th City clinical hospitalRussian Federation

2022

06062025

13196103

2025

Popov A.L., Kolmanovich D.D., Popova N.R., Sorokina S.S., Ivanova O.S., Chukavin N.N., Shcherbakov A.B., Kozlova T.O., Kalashnikova S.A., Ivanov V.K.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://nanojournal.ifmo.ru/jour/article/view/221

Nanoscale cerium dioxide (CeO2, nanoceria) possesses notable redox activity, which is actively used in advanced biomedical applications. The low toxicity, high biocompatibility and antioxidant activity of nanoceria make it a new generation nanozyme with a unique activity. Combination of nanoceria with various biologically active substances results in organic-inorganic nanocomposites possessing enhanced activity. Here, we synthesized a novel organic-inorganic hybrid material (Mil-CeO2) based on 2-(2-carboxylatoethyl)-1,1,1-trimethylhydrazinium and nanoceria, which has an ultra-small particle size, high antioxidant activity and pronounced biological activity. The analysis of cytotoxicity of the composite did not reveal any negative effects on the NCTC L929 mouse fibroblasts at concentrations below 10 mM. It was shown that the nanocomposite did not cause morphological changes in cells, or lead to cell death and mitochondrial membrane potential disruption, while maintaining viability in mouse fibroblasts in vitro. Additionally, we showed that Mil-CeO2 is capable of protecting cells from hydrogen peroxide (H2O2)-induced or radiation-induced oxidative stress.

cerium oxide nanoparticlesnanoceria2-(2-carboxylatoethyl)-111-trimethylhydraziniumtoxicitynanocomposite

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The authors declare that there are no conflicts of interest present.