najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМО10.17586/2220-8054-2024-15-2-300-306najo-31Research ArticleCHEMISTRY AND MATERIALS SCIENCEХИМИЯ И НАУКА О МАТЕРИАЛАХA plasmonic turn-on insulin sensing platform on centimeter scale nanostructure arraysПлатформа плазмонного включения, чувствительная к инсулину, на массивах наноструктур сантиметрового масштабаhttps://orcid.org/0009-0005-2040-2781НгуенТхань Тхи ВанNguyenThanh Thi Van

No.1 Dai Co Viet street, Hanoi 100000

thanh.nguyenthivan@hust.edu.vnSchool of Materials Science and Engineering, Hanoi University of Science and TechnologyViet Nam202431052025152300306Copyright © Nguyen T.T., 20252025Нгуен Т.Т.Nguyen T.T.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/31

We present a plasmonic turn-on biosensing assay for insulin detection via aptamer DNA based on the plasmonic interaction of centimeter scale gold/silver double-layer nanodisk arrays and gold nanoparticles. The large-scale nanostructures were fabricated by laser interference lithography technique. The detecting optical signal-extinction spectra of the system were monitored by UV-visible spectrophotometry. The 3D finite-difference time-domain simulation was used to observe the plasmonic interaction of the sensing system. The platform exhibits an exceptionally large turn-on signal by a 120 nm red shift of the localized surface plasmonic resonance peak, results in the limit detection of 140 pM. The centimeter-scale localized surface plasmon resonance nanostructures combined with turn-on design scheme would offer a promising sensor-on-chip biosensing platform.

Мы представляем плазмонный биосенсорный анализ включения для обнаружения инсулина с помощью аптамерной ДНК, основанный на плазмонном взаимодействии двухслойных массивов нанодисков золота и серебра сантиметрового масштаба и наночастиц золота. Крупномасштабные наноструктуры были изготовлены методом лазерной интерференционной литографии. Детектирующий оптический сигнал - спектры экстинкции системы контролировались методом УФ-видимой спектрофотометрии. Трехмерное моделирование во временной области с конечной разностью использовалось для наблюдения за плазмонным взаимодействием сенсорной системы. Платформа демонстрирует исключительно большой сигнал включения за счет красного смещения на 120 нм пика локализованного поверхностного плазмонного резонанса, что приводит к пределу обнаружения 140 пМ. Наноструктуры локализованного поверхностного плазмонного резонанса сантиметрового масштаба в сочетании со схемой включения могут предложить многообещающую биосенсорную платформу «сенсор на кристалле».

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The authors declare that there are no conflicts of interest present.