najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМО10.17586/2220-8054-2021-12-2-175-181najo-356Research ArticleNANOSYSTEMS: PHYSICS, CHEMISTRY, MATHEMATICSНАНОСИСТЕМЫ: ФИЗИКА, ХИМИЯ, МАТЕМАТИКАAdditive effects of green LED light and cerium oxide nanoparticles on the planarian’s regenerationАддитивные эффекты зеленого светодиодного света и наночастиц оксида церия на регенерацию планарииErmakovA. М.ErmakovA. M.

Institutskaya str., 3, Pushchino, 142290

Institutskaya str., 3, Pushchino, 142290

ao_ermakovy@rambler.ruKamenskikhK. А.KamenskikhK. A.

Institutskaya str., 3, Pushchino, 142290

Institutskaya str., 3, Pushchino, 142290

kristina.kamensk@mail.ruPopovА. L.PopovA. L.

Institutskaya str., 3, Pushchino, 142290

Institutskaya str., 3, Pushchino, 142290

antonpopovleonid@gmail.comErmakovaO. N.ErmakovaO. N.

Institutskaya str., 3, Pushchino, 142290

Institutskaya str., 3, Pushchino, 142290

beoluchi@yandex.ruAfanasyevaV. А.AfanasyevaV. A.

Institutskaya str., 3, Pushchino, 142290

Institutskaya str., 3, Pushchino, 142290

va_vera_afanaseva@mail.ruIvanovV. K.IvanovV. K.

Leninskiy prosp., 31, Moscow, 119991

Leninskiy prosp., 31, Moscow, 119991

van@igic.ras.ruInstitute of Theoretical and Experimental Biophysics of the Russian Academy of SciencesInstitute of Theoretical and Experimental Biophysics of the Russian Academy of SciencesKurnakov Institute of General and Inorganic Chemistry of the Russian Academy of SciencesKurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences202128072025122175181Copyright © Ermakov A.M., Kamenskikh K.A., Popov A.L., Ermakova O.N., Afanasyeva V.A., Ivanov V.K., 20252025Ermakov A.М., Kamenskikh K.А., Popov А.L., Ermakova O.N., Afanasyeva V.А., Ivanov V.K.Ermakov A.M., Kamenskikh K.A., Popov A.L., Ermakova O.N., Afanasyeva V.A., Ivanov V.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/356

Nanotechnology makes it possible to design advanced materials being able to effectively modulate radiation effects on a cell, depending on the radiation intensity, wavelength, and type. Today, one of the most promising UV and X-ray protective biomaterials is nanocrystalline cerium oxide (CeO2), which has a unique redox activity due to its surface reducibility. Meanwhile, the modulating properties of CeO2 nanoparticles when the cells are exposed to visible light remain completely unexplored. Here, we analyzed the impact of CeO2 nanoparticles on the process of planarian regeneration after exposure to low-intensity green LED light. It was found that a one-time exposure (10 or 25 min) of regenerating planarians with low-intensity green light reduced head blastema growth rate by up to 20%. At the same time, the preliminary treatment of planaria with CeO2 nanoparticles in nanomolar concentrations (10-11 M) ensures the restoration of the neoblasts activity and a significant acceleration of blastema regeneration. Thus, we have firstly demonstrated that the planarian regeneration process can be promoted by cerium oxide nanoparticles even under adverse action of low-intensity green light radiation.

Нанотехнологии позволяют создавать передовые материалы, способные эффективно модулировать радиационное воздействие на клетку в зависимости от интенсивности, длины волны и типа излучения. На сегодняшний день одним из наиболее перспективных биоматериалов, защищающих от ультрафиолетового и рентгеновского излучения, является нанокристаллический оксид церия (CeO2), обладающий уникальной окислительно-восстановительной активностью благодаря способности восстанавливаться на поверхности. Между тем, модулирующие свойства наночастиц CeO2 при воздействии на клетки видимого света остаются совершенно неизученными. Проанализировано влияние наночастиц CeO2 на процесс регенерации планарий после воздействия зеленого светодиодного света низкой интенсивности. Установлено, что однократное воздействие (10 или 25 мин) на регенерирующих планарий зеленым светом низкой интенсивности снижало скорость роста головной бластемы до 20 %. В то же время предварительная обработка планарий наночастицами CeO2 в наномолярных концентрациях (10-11 М) обеспечивает восстановление активности необластов и значительное ускорение регенерации бластемы. Таким образом, мы впервые показали, что наночастицы оксида церия могут стимулировать процесс регенерации планарии даже при неблагоприятном воздействии низкоинтенсивного излучения зеленого света.

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The authors declare that there are no conflicts of interest present.