najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМО10.17586/2220-8054-2019-10-1-92-101najo-537Research ArticleCHEMISTRY AND MATERIALS SCIENCEХИМИЯ И НАУКА О МАТЕРИАЛАХPVP-stabilized tungsten oxide nanoparticles inhibit proliferation of NCTC L929 mouse fibroblasts via induction of intracellular oxidative stressPopovA. L.

Pushchino, Moscow region, 142290

antonpopovleonid@gmail.comErmakovA. M.

Pushchino, Moscow region, 142290

ao_ermakovy@rambler.ruShekunovaT. O.

Moscow, 119991

taisia.shekunova@yandex.ruShcherbakovA. B.

Kyiv, D0368 

carotene@igic.ras.ruErmakovaO. N.

Pushchino, Moscow region, 142290

beoluchi@yandex.ruIvanovaO. S.

Moscow, 119991

runetta05@mail.ruPopovaN. R.

Pushchino, Moscow region, 142290

nellipopovaran@gmail.comBaranchikovA. Ye.

Moscow, 119991

a.baranchikov@yandex.ruIvanovV. K.

Moscow, 119991

van@igic.ras.ruInstitute of Theoretical and Experimental Biophysics, Russian Academy of SciencesRussian FederationLomonosov Moscow State University ; Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of SciencesRussian FederationZabolotny Institute of Microbiology and Virology, National Academy of Sciences of UkraineUkraineKurnakov Institute of General and Inorganic Chemistry, Russian Academy of SciencesRussian Federation20190608202510192101Copyright © Popov A.L., Ermakov A.M., Shekunova T.O., Shcherbakov A.B., Ermakova O.N., Ivanova O.S., Popova N.R., Baranchikov A.Y., Ivanov V.K., 20252025Popov A.L., Ermakov A.M., Shekunova T.O., Shcherbakov A.B., Ermakova O.N., Ivanova O.S., Popova N.R., Baranchikov A.Y., Ivanov V.K.Popov A.L., Ermakov A.M., Shekunova T.O., Shcherbakov A.B., Ermakova O.N., Ivanova O.S., Popova N.R., Baranchikov A.Y., Ivanov V.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/537

In this study, photochromic PVP-stabilized tungsten oxide nanoparticles (WO3-x NPs) were shown to exhibit a dose-dependent cytotoxic effect on mouse fibroblasts in vitro. WO3-x NPs reduce viability and proliferative activity of the cells via induction of intracellular oxidative stress leading to apoptosis and cell death. WO3-x NPs modulate the mRNA expression of a wide range of genes responsible for oxidative stress and the cell redox-system.

tungsten oxide nanoparticlescytotoxicityapoptosisfibroblastsThis study was funded by Russian Science Foundation (project 18-73-10150).ReferencesFirouzi M., Poursalehi R., et al. Chitosan coated tungsten trioxide nanoparticles as a contrast agent for X-ray computed tomography. Int. J. Biol. Macromol., 2017, 98, P. 479–485.Firouzi M., Poursalehi R., et al. Chitosan coated tungsten trioxide nanoparticles as a contrast agent for X-ray computed tomography. Int. J. Biol. Macromol., 2017, 98, P. 479–485.Popov A.L., Savintseva I.V., et al. Cytotoxicity analysis of gadolinium doped cerium oxide nanoparticles on human mesenchymal stem cells. Nanosyst. Phys. Chem. Math., 2018, 9 (3), P. 430–438.Popov A.L., Savintseva I.V., et al. Cytotoxicity analysis of gadolinium doped cerium oxide nanoparticles on human mesenchymal stem cells. Nanosyst. Phys. Chem. Math., 2018, 9 (3), P. 430–438.Popov A.L., Shcherbakov A.B., et al. Cerium dioxide nanoparticles as third-generation enzymes (Nanozymes). Nanosyst. Phys. Chem. Math., 2017, 8 (6), P. 760–781.Popov A.L., Shcherbakov A.B., et al. Cerium dioxide nanoparticles as third-generation enzymes (Nanozymes). Nanosyst. Phys. Chem. Math., 2017, 8 (6), P. 760–781.Popova N.R., Popov A.L., Shcherbakov A.B., Ivanov V.K. Layer-by-layer capsules as smart delivery systems of CeO2 nanoparticle-based theranostic agents. Nanosyst. Phys. Chem. Math., 2017, 8 (2), P. 282–289.Popova N.R., Popov A.L., Shcherbakov A.B., Ivanov V.K. Layer-by-layer capsules as smart delivery systems of CeO2 nanoparticle-based theranostic agents. Nanosyst. Phys. Chem. Math., 2017, 8 (2), P. 282–289.Popov A.L., Popova N.R., et al. Cerium oxide nanoparticles stimulate proliferation of primary mouse embryonic fibroblasts in vitro. Mater. Sci. Eng., C, 2016, 68, P. 406–413.Popov A.L., Popova N.R., et al. Cerium oxide nanoparticles stimulate proliferation of primary mouse embryonic fibroblasts in vitro. Mater. Sci. Eng., C, 2016, 68, P. 406–413.Hosseini F., Rasuli R., Jafarian V. Immobilized WO3 nano-particles on graphene oxide as a photo-induced antibacterial agent against UV resistant Bacillus Pumilus. J. Phys. D: Appl. Phys., 2018, 51 (14), 145403.Hosseini F., Rasuli R., Jafarian V. Immobilized WO3 nano-particles on graphene oxide as a photo-induced antibacterial agent against UV resistant Bacillus Pumilus. J. Phys. D: Appl. Phys., 2018, 51 (14), 145403.Hariharan V., Radhakrishnan S., et al. Synthesis of polyethylene glycol (PEG) assisted tungsten oxide (WO3) nanoparticles for L-dopa bio-sensing applications. Talanta, 2011, 85 (4), P. 2166–2174.Hariharan V., Radhakrishnan S., et al. Synthesis of polyethylene glycol (PEG) assisted tungsten oxide (WO3) nanoparticles for L-dopa bio-sensing applications. Talanta, 2011, 85 (4), P. 2166–2174.Deng K., Hou Z., et al. Enhanced Antitumor Efficacy by 808 nm Laser-Induced Synergistic Photothermal and Photodynamic Therapy Based on a Indocyanine-Green-Attached W18O49 Nanostructure. Adv. Funct. Mater., 2015, 25 (47), P. 7280–7290.Deng K., Hou Z., et al. Enhanced Antitumor Efficacy by 808 nm Laser-Induced Synergistic Photothermal and Photodynamic Therapy Based on a Indocyanine-Green-Attached W18O49 Nanostructure. Adv. Funct. Mater., 2015, 25 (47), P. 7280–7290.Chen Z., Wang Q., et al. Ultrathin PEGylated W18O49 nanowires as a new 980 nm-laser-driven photothermal agent for efficient ablation of cancer cells in vivo. Adv. Mater., 2013, 25 (14), P. 2095–2100.Chen Z., Wang Q., et al. Ultrathin PEGylated W18O49 nanowires as a new 980 nm-laser-driven photothermal agent for efficient ablation of cancer cells in vivo. Adv. Mater., 2013, 25 (14), P. 2095–2100.Sharker S.Md., Kim S.M., et al. Functionalized biocompatible WO3 nanoparticles for triggered and targeted in vitro and in vivo photothermal therapy. J. Control. Release, 2015, 217, P. 211–220.Sharker S.Md., Kim S.M., et al. Functionalized biocompatible WO3 nanoparticles for triggered and targeted in vitro and in vivo photothermal therapy. J. Control. Release, 2015, 217, P. 211–220.Zhou Z., Kong B., et al. Tungsten Oxide Nanorods: An Efficient Nanoplatform for Tumor CT Imaging and Photothermal Therapy. Sci. Rep., 2014, 4, 3653.Zhou Z., Kong B., et al. Tungsten Oxide Nanorods: An Efficient Nanoplatform for Tumor CT Imaging and Photothermal Therapy. Sci. Rep., 2014, 4, 3653.Liu J., Han J., et al. In vivo near-infrared photothermal therapy and computed tomography imaging of cancer cells using novel tungstenbased theranostic probe. Nanoscale, 2014, 6 (11), P. 5770–5776.Liu J., Han J., et al. In vivo near-infrared photothermal therapy and computed tomography imaging of cancer cells using novel tungstenbased theranostic probe. Nanoscale, 2014, 6 (11), P. 5770–5776.Liu P., Wang Y., et al. Ultrasmall WO3−x@γ-poly-l-glutamic Acid Nanoparticles as a Photoacoustic Imaging and Effective PhotothermalEnhanced Chemodynamic Therapy Agent for Cancer. ACS Appl. Mater. Interfaces, 2018, 10 (45), P. 38833–3884.Liu P., Wang Y., et al. Ultrasmall WO3−x@γ-poly-l-glutamic Acid Nanoparticles as a Photoacoustic Imaging and Effective PhotothermalEnhanced Chemodynamic Therapy Agent for Cancer. ACS Appl. Mater. Interfaces, 2018, 10 (45), P. 38833–3884.AbuMousa R.A., Baig U., et al. Photo-catalytic Killing of HeLa Cancer Cells Using Facile Synthesized Pure and Ag Loaded WO3 Nanoparticles. Sci. Rep., 2018, 8, 15224.AbuMousa R.A., Baig U., et al. Photo-catalytic Killing of HeLa Cancer Cells Using Facile Synthesized Pure and Ag Loaded WO3 Nanoparticles. Sci. Rep., 2018, 8, 15224.Popov A., Zholobak N., et al. Photo-induced toxicity of tungsten oxide photochromic nanoparticles. J. Photochem. Photobiol. B, 2018, 178, P. 395–403.Popov A., Zholobak N., et al. Photo-induced toxicity of tungsten oxide photochromic nanoparticles. J. Photochem. Photobiol. B, 2018, 178, P. 395–403.

The authors declare that there are no conflicts of interest present.