najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМО10.17586/2220-8054-2021-12-6-749-762najo-573Research ArticleCHEMISTRY AND MATERIALS SCIENCEХИМИЯ И НАУКА О МАТЕРИАЛАХNovel gold nanoparticle-protein-semiconductor quantum dot hybrid system: synthesis, characterization and application in cancer cell imagingНовая гибридная система наночастицы золота – белок – полупроводниковые квантовые точки: синтез, характеристика и применение для визуализации раковых клетокSarmaRunjunSarmaRunjun

Sector-36, Chandigarh.

runjun2018chd@gmail.comKumarDasMonojKumarDasMonoj

Assam-784028.

SaikiaLakshiSaikiaLakshi

Jorhat, Assam-785006.

SaikiaRatulSaikiaRatul

Jorhat, Assam-785006.

Department of Physics, Mehr Chand Mahajan DAV College for WomenIndiaCancer Genetics and Chemoprevention Research Group, Department of Molecular Biology and Biotechnology, Tezpur UniversityIndiaAdvanced Materials Group, Material Science and Technology Division, CSIR-North East Institute of Science and TechnologyIndiaBiological Science and Technology Division, CSIR-North East Institute of Science and TechnologyIndia202107082025126749762Copyright © Sarma R., KumarDas M., Saikia L., Saikia R., 20252025Sarma R., KumarDas M., Saikia L., Saikia R.Sarma R., KumarDas M., Saikia L., Saikia R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/573

Formation of a hybrid system by metallic nanoparticle, protein (or peptide), and a semiconductor QD can be a new and alternate material which may be used for biological applications including cancer cell detection and treatment. Herein, we report on the colloidal synthesis of metal-protein-QD hybrid system considering Au NPs, CdSe QDs, BSA and Lysozyme protein. We demonstrate the structural, optical and vibrational properties of Au-CdSe, Au-BSA-CdSe and Au-Lysozyme-CdSe hybrid systems following their use as cancer cell markers. The study of photoluminescence spectra reveals the predominance fluorescence resonance energy transfer (FRET) between CdSe QDs and Au NPs, Au-BSA and Au-Lysozyme complex. The energy transfer efficiency between QDs donor and the Au NPs, Au-BSA, Au-Lysozyme acceptors are estimated to be 46%, 94% and 64%; respectively. Fluorescence imaging results represent high biocompatibility and fluorescent behavior of the QDs and its hybrid system in the MDA-MB-231 breast cancer cells. The calculation of corrected total counts of fluorescence (CTCF) predicts the higher uptake of CdSe QDs as compared to the Au-QDs, Au-BSA-QDs, Au-Lysozyme-QDs by the cells. The significantly varied zeta potential values of the hybrid systems influence the cellular uptake processes. The fabrication of biocompatible (water soluble, biologically stable, having bioconjugation capability, low cytotoxic to the normal cells, fluorescent in biological environment) Au-CdSe, Au-protein-CdSe hybrid systems would open up an alternative strategy in nanobiotechnology, due to their special physical, optical as well as chemical properties.

Формирование гибридной системы из металлических наночастиц, белков (или пептидов) и полупроводниковых квантовых точек (QD) может быть новым, альтернативным материалом, который можно использовать для биологических приложений, включая обнаружение и лечение раковых клеток. В работе сообщается о коллоидном синтезе гибридной системы металл – белок – QD, включающих наночастицы Au, CdSe в качестве квантовых точек, BSA и белок лизоцима. Мы демонстрируем структурные, оптические и колебательные свойства гибридных систем Au – CdSe, Au – BSA – CdSe и Au – лизоцим – CdSe после их использования в качестве маркеров раковых клеток. Изучение спектров фотолюминесценции выявило преобладание резонансного переноса энергии флуоресценции (FRET) между QD CdSe и наночастицами Au, комплексом Au- BSA и Au-лизоцим. Эффективность переноса энергии между донором (QD) и акцепторами (НЧ Au, Au-BSA, Au-лизоцим) оценивается в 46%, 94% и 64%; соответственно. Результаты флуоресцентной визуализации демонстрируют высокую биосовместимость и флуоресцентное поведение QD и гибридных систем в клетках рака молочной железы MDA-MB-231. Расчет скорректированного общего количества флуоресценции предсказывает более высокое поглощение клетками QD CdSe по сравнению с Au – QD, Au – BSA – QD, Au – лизоцим –QD. Значительно различающиеся значения дзета-потенциала гибридных систем влияют на процессы клеточного поглощения. Изготовление биосовместимых (водорастворимых, биологически стабильных, обладающих способностью к биоконъюгации, низкой цитотоксичности по отношению к нормальным клеткам, флуоресцирующих в биологической среде) гибридных систем Au – CdSe, Au – белок – CdSe открыло бы альтернативную стратегию в нанобиотехнологии благодаря их особые физические, оптические и химические свойства.

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The authors declare that there are no conflicts of interest present.