najoNanosystems: Physics, Chemistry, MathematicsНаносистемы: физика, химия, математика2220-80542305-7971Университет ИТМО10.17586/2220-8054-2017-8-4-531-534najo-780Research ArticleCHEMISTRY AND MATERIALS SCIENCEХИМИЯ И НАУКА О МАТЕРИАЛАХAntibacterial activity of Amikacin-immobilized detonation nanodiamondAntibacterial activity of Amikacin-immobilized detonation nanodiamondSolomatinA. S.SolomatinA. S.

Ryazan; Moscow

Ryazan; Moscow

solo-msu@yandex.ruYakovlevR. Yu.YakovlevR. Yu.

Ryazan; Moscow

Ryazan; Moscow

yarules@yandex.ruEfremenkovaO. V.EfremenkovaO. V.

Moscow

Moscow

ovefr@yandex.ruSumarukovaI. G.SumarukovaI. G.

Moscow

Moscow

noks59@yandex.ruKulakovaI. I.KulakovaI. I.

Moscow

Moscow

inna-kulakova@yandex.ruLisichkinG. V.LisichkinG. V.

Moscow

Moscow

lisich@petrol.chem.msu.ruRyazan State Medical University; Lomonosov Moscow State UniversityRyazan State Medical University; Lomonosov Moscow State UniversityRyazan State Medical University; Lomonosov Moscow State University; Vernadsky Institute of Geochemistry and Analytical Chemistry of Russian Academ of SciencesRyazan State Medical University; Lomonosov Moscow State University; Vernadsky Institute of Geochemistry and Analytical Chemistry of Russian Academ of SciencesGause Institute of New AntibioticsGause Institute of New AntibioticsLomonosov Moscow State UniversityLomonosov Moscow State University20171308202584531534Copyright © Solomatin A.S., Yakovlev R.Y., Efremenkova O.V., Sumarukova I.G., Kulakova I.I., Lisichkin G.V., 20252025Solomatin A.S., Yakovlev R.Y., Efremenkova O.V., Sumarukova I.G., Kulakova I.I., Lisichkin G.V.Solomatin A.S., Yakovlev R.Y., Efremenkova O.V., Sumarukova I.G., Kulakova I.I., Lisichkin G.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://nanojournal.ifmo.ru/jour/article/view/780

   Detonation nanodiamonds (NDs) with chlorinated (ND–Cl) and carboxylated (ND–COOH) surfaces were obtained. The broad-spectrum antibiotic Amikacin (Amik) was covalently grafted to the chlorinated surface (ND–Amik) and immobilized by adsorption to carboxylated surface (Amik/ND–COOH). Biological testing in vitro showed the presence of antibacterial activity of the obtained samples against Staphylococcus aureus FDA P209 and Escherichia coli ATCC 25922, close to activity of free amikacin. It was revealed that to maintain antibacterial activity of the samples after their preliminary treatment, important factors such as the use of antioxidants (hydrosulfite and sodium citrate) and lyophilization were necessary.

   Detonation nanodiamonds (NDs) with chlorinated (ND–Cl) and carboxylated (ND–COOH) surfaces were obtained. The broad-spectrum antibiotic Amikacin (Amik) was covalently grafted to the chlorinated surface (ND–Amik) and immobilized by adsorption to carboxylated surface (Amik/ND–COOH). Biological testing in vitro showed the presence of antibacterial activity of the obtained samples against Staphylococcus aureus FDA P209 and Escherichia coli ATCC 25922, close to activity of free amikacin. It was revealed that to maintain antibacterial activity of the samples after their preliminary treatment, important factors such as the use of antioxidants (hydrosulfite and sodium citrate) and lyophilization were necessary.

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The authors declare that there are no conflicts of interest present.