New superparamagnetic fluorescent Fe@C-C  5  ON  2  H  10  -Alexa Fluor 647 nanoparticles for biological applications

A. S. Garanina; I. I. Kireev; I. B. Alieva; A. G. Majouga; V. A. Davydov; S. Murugesan; V. N. Khabashesku; V. N. Agafonov; R. E. Uzbekov

doi:10.17586/2220-8054-2018-9-1-120-122

New superparamagnetic fluorescent Fe@C-C₅ON₂H₁₀-Alexa Fluor 647 nanoparticles for biological applications

A. S. Garanina, I. I. Kireev, I. B. Alieva, A. G. Majouga, V. A. Davydov, S. Murugesan, V. N. Khabashesku, V. N. Agafonov, R. E. Uzbekov

https://doi.org/10.17586/2220-8054-2018-9-1-120-122

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Abstract

The structure and physical properties of superparamagnetic Fe@C nanoparticles (Fe@C NPs) as well as their uptake by living cells and behavior inside the cell were investigated. Magnetic capacity of Fe@C NPs was compared with Fe7C3@C NPs investigated in our previous work, and showed higher value of magnetic saturation, 75 emu.g−1 (75 Am2·kg−1), against 54 emu.g−1 (54 Am2·kg−1) for Fe7C3@C. The surface of Fe@C NPs was alkylcarboxylated and further aminated for covalent linking to the molecules of fluorochrome Alexa Fluor 647. Fluorescent Fe@C-C5ON2H10-Alexa Fluor 647 NPs (Fe@C-Alexa NPs) were incubated with HT1080 human fibrosarcoma cells and investigated using fluorescent, confocal laser scanning and transmission electron microscopy. No toxic effect on the cell physiology was observed. In a magnetic field, the NPs became aligned along the magnetic lines inside the cells.