New superparamagnetic fluorescent Fe@C-C  5  ON  2  H  10  -Alexa Fluor 647 nanoparticles for biological applications

doi:10.17586/2220-8054-2018-9-1-120-122

New superparamagnetic fluorescent Fe@C-C₅ON₂H₁₀-Alexa Fluor 647 nanoparticles for biological applications

A. Garanina, I. Kireev, I. Alieva, A. Majouga, V. Davydov, S. Murugesan, V. Khabashesku, V. Agafonov, R. Uzbekov

https://doi.org/10.17586/2220-8054-2018-9-1-120-122

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Аннотация

The structure and physical properties of superparamagnetic Fe@C nanoparticles (Fe@C NPs) as well as their uptake by living cells and behavior inside the cell were investigated. Magnetic capacity of Fe@C NPs was compared with Fe7C3@C NPs investigated in our previous work, and showed higher value of magnetic saturation, 75 emu.g−1 (75 Am2·kg−1), against 54 emu.g−1 (54 Am2·kg−1) for Fe7C3@C. The surface of Fe@C NPs was alkylcarboxylated and further aminated for covalent linking to the molecules of fluorochrome Alexa Fluor 647. Fluorescent Fe@C-C5ON2H10-Alexa Fluor 647 NPs (Fe@C-Alexa NPs) were incubated with HT1080 human fibrosarcoma cells and investigated using fluorescent, confocal laser scanning and transmission electron microscopy. No toxic effect on the cell physiology was observed. In a magnetic field, the NPs became aligned along the magnetic lines inside the cells.