UV-tuning the redox properties of nanoscale cerium dioxide and its enzyme conjugates
M. M. Sozarukova,
A. D. Filippova,
D.-M. V. Ratova,
I. V. Mikheev,
E. V. Proskurnina,
A. E. Baranchikov,
V. K. Ivanov https://doi.org/10.17586/2220-8054-2025-16-6-791-801
Abstract
This study investigated the redox properties of cerium oxide nanoparticles (CeO2 NPs) and their conjugates with superoxide dismutase (SOD) or horseradish peroxidase (HRP) as well as the UV-induced modulation of these properties. UV exposure non-monotonically decreased the SOD-like property of the bare CeO2 NPs. The CeO2 conjugates with enzymes were analyzed both immediately after preparation and after being aged for 3 h. Chemiluminescence assays showed the synergistic effect for the CeO2-SOD conjugates which showed high SOD activity. Additionally, CeO2 NPs enhanced the stability of the conjugated SOD under UV exposure thus demonstrating a photoprotective function. The CeO2-HRP conjugates demonstrated lower prooxidant activity compared to the bare enzyme, however higher stability under UV irradiation. The effect of UV radiation on CeO2-HRP conjugates was found to be multidirectional and depended on the incubation time f the CeO2 NPs with the enzyme. The results demonstrated that CeO2-enzyme conjugates offer tunable dual functionality and UV light could be an important parameter affecting their redox properties. The latter be taken into account for designing advanced cosmeceutical formulations.
Keywords
nanoceria,
superoxide dismutase,
peroxidase,
UV irradiation,
enzyme,
conjugate,
redox modulation,
photoprotection
Supporting agencies: This study was supported by the Russian Science Foundation (Project no. 24-13- 00370).
About the Authors
M. M. Sozarukova
Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences
Russian Federation
Russian Federation
Madina M. Sozarukova
Leninskii prospect 31, Moscow, 119991
A. D. Filippova
Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences
Russian Federation
Russian Federation
Arina D. Filippova
Leninskii prospect 31, Moscow, 119991
D.-M. V. Ratova
M. V. Lomonosov Moscow State University
Russian Federation
Russian Federation
Daria-Maria V. Ratova – Analytical Chemistry Division, Chemistry Department
d. 1, Str. 3, Lenin Hills, GSP-1, Moscow, 119234
I. V. Mikheev
M. V. Lomonosov Moscow State University
Russian Federation
Russian Federation
Ivan V. Mikheev – Analytical Chemistry Division, Chemistry Department
d. 1, Str. 3, Lenin Hills, GSP-1, Moscow, 119234
E. V. Proskurnina
Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences
Russian Federation
Russian Federation
Elena V. Proskurnina
Leninskii prospect 31, Moscow, 119991
A. E. Baranchikov
Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences
Russian Federation
Russian Federation
Alexander E. Baranchikov
Leninskii prospect 31, Moscow, 119991
V. K. Ivanov
Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences
Russian Federation
Russian Federation
Vladimir K. Ivanov
Leninskii prospect 31, Moscow, 119991
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Review
For citations:
Sozarukova M.M., Filippova A.D., Ratova D.V., Mikheev I.V., Proskurnina E.V., Baranchikov A.E., Ivanov V.K. UV-tuning the redox properties of nanoscale cerium dioxide and its enzyme conjugates. Nanosystems: Physics, Chemistry, Mathematics. 2025;16(6):791-801. https://doi.org/10.17586/2220-8054-2025-16-6-791-801
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