УФ-модуляция редокс-свойств нанодисперсного диоксида церия и его конъюгатов с ферментами

В настоящей работе исследовано влияние УФ-облучения на редокс-свойства нанодисперсного диоксида церия и его конъюгатов с ферментами – супероксиддисмутазой (СОД) и пероксидазой из корней хрена. По данным хемилюминесцентного анализа, наночастицы СеО₂ обладают СОД-подобной активностью, при этом радикал-перехватывающие свойства конъюгатов СеО₂ с ферментом СОД значительно усиливаются за счет синергетического эффекта. Установлено, что воздействие УФ-облучения снижает СОД-подобную активность наночастиц СеО₂ и их конъюгатов с СОД. В составе конъюгатов нанодисперсный диоксид церия повышает устойчивость СОД к окислительной деструкции, вызванной УФ-облучением, что свидетельствует о фотопротекторных свойствах наночастиц СеО₂. Конъюгаты нанодисперсного СеО₂ с пероксидазой из корней хрена проявляют значительно меньшую прооксидатную активность, но большую фотостабильность по сравнению с индивидуальным ферментом. Влияние УФ-облучения на прооксидантные свойства конъюгатов СеО₂ с пероксидазой из корней хрена носило разнонаправленный характер и зависело от времени взаимодействия диоксида церия с белком. Результаты подтверждают, что конъюгаты наночастиц СеО₂ с ферментами обладают активностью, модулируемой в том числе УФ-излучением, что следует учитывать при разработке современных космецевтических препаратов.

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