Nanosystems: Phys. Chem. Math., 2025, 16 (1), 22–29
Peroxidase-like activity of photochromic PVP-stabilized tungsten oxide nanoparticles: assessment by independent chemiluminescent and colorimetric assays
Matvei A. Popkov – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; ma_popkov@igic.ras.ru
EkaterinaD. Sheichenko – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow; HSE University, Moscow, Russia; edsheychenko@edu.hse.ru
ArinaD. Filippova – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; arifilippova@yandex.ru
Ilya V. Tronev – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow; HSE University, Moscow, Russia; ivtronev@edu.hse.ru
Kristina N. Novoselova – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow; HSE University, Moscow, Russia; knnovoselova@edu.hse.ru
Evelina A. Trufanova – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow; HSE University, Moscow, Russia; eatrufanova_1@edu.hse.ru
Darya N. Vasilyeva – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow; HSE University, Moscow, Russia; Dnvasileva_1@edu.hse.ru
Maria R. Protsenko – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow; HSE University, Moscow, Russia; mrprotsenko@edu.hse.ru
Madina M. Sozarukova – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; s_madinam@bk.ru
Alexander E. Baranchikov – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow; HSE University, Moscow, Russia; a.baranchikov@yandex.ru
Vladimir K. Ivanov – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow; HSE University, Moscow, Russia; van@igic.ras.ru
Corresponding author: M. A. Popkov, ma popkov@igic.ras.ru
PACS 68.65.-k, 78.67.-n, 82.65.+r
ABSTRACT In this work, peroxidase-like activity of ultrasmall tungsten oxide nanoparticles including those stabilized with polyvinylpyrrolidone (PVP) was assessed using two independent approaches: the colorimetric method, which is based on the analysis of 3,3’,5,5’-tetramethylbenzidine (TMB) oxidation, and the luminolenhanced chemiluminescence method. It was demonstrated that bare tungsten oxide nanoparticles effectively catalyze the decomposition of hydrogen peroxide. In turn, the PVP-stabilized nanoparticles possess lower catalytic activity, which can be attributed to a decrease in the number of available active centres on the nanoparticle surface.
KEYWORDS nanomaterials, nanozymes, sols, photochromic materials, chemiluminescence, TMB, peroxidase-like activity
ACKNOWLEDGEMENTS The publication was prepared within the framework of the Academic Fund Program at HSE University (grant 24-00-036, Enzyme-like activity of nanodispersed inorganic materials). The studies were carried out on the equipment of the Joint Scientific Center for Physical Research Methods of the Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.
FOR CITATION Popkov M.A., Sheichenko E.D., Filippova A.D., Tronev I.V., Novoselova K.N., Trufanov E.A., Vasilyeva D.N., Protsenko M.R., Sozarukova M.M., Baranchikov A.E., Ivanov V.K. Peroxidase-like activity of photochromic PVP-stabilized tungsten oxide nanoparticles: assessment by independent chemiluminescent and colorimetric assays. Nanosystems: Phys. Chem. Math., 2025, 16 (1), 22–29.
[In Russian] M. А. Попков, Е. Д. Шейченко, А. Д. Филиппова, И. В. Тронев, К. Н. Новоселова, Э. А. Труфанова, Д. Н. Васильева, М. Р. Проценко, М. М. Созарукова, А. Е. Баранчиков, В. К. Иванов
Пероксидазоподобная активность фотохромных наночастиц оксида вольфрама, стабилизированных ПВП: независимый анализ хемилюминесцентным и колориметрическим методами
УДК 546.78-31:544.47
АННОТАЦИЯ В данной работе пероксидазоподобную активность ультрамелких наночастиц оксида вольфрама, в том числе стабилизированных поливинилпирролидоном (ПВП), оценивали с помощью двух независимых подходов: колориметрического метода, основанного на анализе окисления 3,3′,5,5′-тетраметилбензидина (ТМБ), и метода хемилюминесценции с использованием люминола в качестве молекулы-зонда. Было показано, что наночастицы нестабилизированного оксида вольфрама эффективно катализируют разложение пероксида водорода. В свою очередь, наночастицы, стабилизированные ПВП, обладают меньшей каталитической активностью, что можно объяснить уменьшением количества доступных активных центров на поверхности наночастиц.
КЛЮЧЕВЫЕ СЛОВА наноматериалы, нанозимы, золи, фотохромные материалы, хемилюминесценция, ТМБ, пероксидазоподобная активность
