14

Nanosystems: Phys. Chem. Math., 2022, 13 (1), 96–103

Synthesis and biocompatibility study of ceria-mildronate nanocomposite in vitro

A. L. Popov – Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 142290, Russia; antonpopovleonid@gmail.com
D.D. Kolmanovich – Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 142290, Russia; kdd100996@mail.ru
N. R. Popova – Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 142290, Russia; nellipopovaran@gmail.com
S. S. Sorokina – Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 142290, Russia; sorokinasvetlana.iteb@gmail.com
O. S. Ivanova – Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, 119991, Russia; runetta05@mail.ru
N. N. Chukavin – Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 142290; Moscow Region State University, 141014, Moscow, Russia; chukavinnik@gmail.com
A. B. Shcherbakov – Zabolotny Institute of Microbiology and Virology, Kyiv D0368, Ukraine; ceroform@gmail.com
T.O. Kozlova – Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, 119991, Russia; tasiok@mail.ru
S. A. Kalashnikova – 40th City clinical hospital, Ekaterinburg, 620102, Russia; kalashnikova.sana@mail.ru
V. K. Ivanov – Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, 119991, Russia; van@igic.ras.ru

Corresponding author: A. L. Popov, antonpopovleonid@gmail.com

DOI 10.17586/2220-8054-2022-13-1-96-103
PACS 68.65.-k, 81.20.-n, 82.70. Dd, 87.80.-y

ABSTRACT Nanoscale cerium dioxide (CeO2, nanoceria) possesses notable redox activity, which is actively used in advanced biomedical applications. The low toxicity, high biocompatibility and antioxidant activity of nanoceria make it a new generation nanozyme with a unique activity. Combination of nanoceria with various biologically active substances results in organic-inorganic nanocomposites possessing enhanced activity. Here, we synthesized a novel organic-inorganic hybrid material (Mil-CeO2) based on 2-(2-carboxylatoethyl)-1,1,1-trimethylhydrazinium and nanoceria, which has an ultra-small particle size, high antioxidant activity and pronounced biological activity. The analysis of cytotoxicity of the composite did not reveal any negative effects on the NCTC L929 mouse fibroblasts at concentrations below 10 mM. It was shown that the nanocomposite did not cause morphological changes in cells, or lead to cell death and mitochondrial membrane potential disruption, while maintaining viability in mouse fibroblasts in vitro. Additionally, we showed that Mil-CeO2 is capable of protecting cells from hydrogen peroxide (H2O2)-induced or radiation-induced oxidative stress.

KEYWORDS cerium oxide nanoparticles, nanoceria, 2- (2-carboxylatoethyl) -1,1,1-trimethylhydrazinium, toxicity, nanocomposite

ACKNOWLEDGEMENTS The work was supported by the Russian Science Foundation (project 20-74-00086). The research was carried out using the equipment of MSU Shared Research Equipment Center “Technologies for obtaining new nanostructured materials and their complex study” and purchased by MSU in the frame of the Equipment Renovation Program (National Project ”Science”) and in the frame of the MSU Program of Development.

FOR CITATION Popov A.L., Kolmanovich D.D., Popova N.R., Sorokina S.S., Ivanova O.S., Chukavin N.N., Shcherbakov A.B., Kozlova T.O., Kalashnikova S.A., Ivanov V.K. Synthesis and biocompatibility study of ceriamildronate nanocomposite in vitro. Nanosystems: Phys. Chem. Math., 2022, 13 (1), 96–103.

Download

Comments are closed.