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Nanosystems: Phys. Chem. Math., 2022, 13 (3), 308–313

CeO2-calcein nanoconjugate protective action against H2O2-induced oxidative stress in vitro

Nikita N. Chukavin – Institute of Theoretical and Experimental Biophysics, Pushchino; Moscow Region State University, Moscow, Russia; chukavinnik@gmail.com
Anton L. Popov – Institute of Theoretical and Experimental Biophysics, Pushchino, Russia; antonpopovleonid@gmail.com
Alexander B. Shcherbakov – Zabolotny Institute of Microbiology and Virology, Kyiv, Ukraine; ceroform@gmail.com
Olga S. Ivanova – Kurnakov Institute of General and Inorganic Chemistry, Moscow, Russia; runetta05@mail.ru
Arina D. Filippova – Kurnakov Institute of General and Inorganic Chemistry, Moscow, Russia
Vladimir K. Ivanov – Kurnakov Institute of General and Inorganic Chemistry, Moscow, Russia; van@igic.ras.ru

Corresponding author: Vladimir K. Ivanov, van@igic.ras.ru

DOI 10.17586/2220-8054-2022-13-3-308-313

PACS 68.65.-k, 81.20.-n, 82.70. Dd, 87.80.-y

ABSTRACT We studied cerium oxide-calcein nanoconjugate, which is capable of providing intracellular detection and simultaneous inactivation of reactive oxygen species (ROS). The synthesized nanoconjugate is easily uptaken by human mesenchymal stem cells (MSCs) and demonstrates antioxidant properties, protecting cells from H2O2-induced oxidative stress in vitro. Cerium oxide-calcein nanoconjugate neutralizes hydrogen peroxide, meanwhile releasing brightly fluorescent calcein from its surface, which is easily detected by fluorimeter or fluorescent microscope. This nanoconjugate is biocompatible and non-toxic to MSCs in concentrations below 2 mM. Such a theranostic agent can be considered as a promising tool for tracking the redox status of human MSCs in vivo.

KEYWORDS cerium oxide, calcein, bioimaging, cell uptake, oxidative stress, theranostics

ACKNOWLEDGEMENTS The work was supported by the Russian Science Foundation (project 20-74-00086).

FOR CITATION Chukavin N.N., Popov A.L., Shcherbakov A.B., Ivanova O.S., Filippova A.D., Ivanov V.K. CeO2-calcein nanoconjugate protective action against H2O2-induced oxidative stress in vitro. Nanosystems: Phys. Chem. Math., 2022, 13 (3), 308–313.

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