NANOSYSTEMS: PHYSICS, CHEMISTRY, MATHEMATICS, 2019, 10 (5), P. 564–572
Cerium oxide nanoparticles provide radioprotective effects upon X-ray irradiation by modulation of gene expression
N. R. Popova – Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia; nellipopovaran@gmail.com
T. O. Shekunova – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia; taisia.shekunova@yandex.ru
A. L. Popov – Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia; antonpopovleonid@gmail.com
I. I. Selezneva – Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia; selezneva_i@mail.ru
V. K. Ivanov – Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia; van@igic.ras.ru
Nanocrystalline cerium dioxide is known as a unique redox active nanomaterial. Cerium dioxide is considered as the basis for future biomedical preparations, including radioprotectors. In the framework of this study, we synthesized citrate-stabilized CeO2 nanoparticles and carried out a comprehensive in vitro assessment of their radioprotective properties on a NCTC L929 murine fibroblast culture. It was shown that CeO2 nanoparticles ensure the survival of murine fibroblasts, even after high-dose X-ray irradiation, reducing the number of dead cells in the culture and modulating the mRNA level of the key antioxidant enzymes – superoxide dismutase 1 (SOD1) and superoxide dismutase 2 (SOD2). The results obtained confirm the potential for studying the properties of CeO2 nanoparticles as basic materials for designing new efficient and safe preparations for protection against ionizing radiation.
Keywords: cerium oxide nanoparticles, radioprotection, cytotoxicity, X-ray, ionizing radiation, fibroblasts.
PACS 68.65.k, 81.20.n, 82.70.Dd, 87.80.-y
DOI 10.17586/2220-8054-2019-10-5-564-572
