NANOSYSTEMS: PHYSICS, CHEMISTRY, MATHEMATICS, 2016, 7 (1), P. 158–160
Magnet-induced behavior of iron carbide (Fe7C3@C) nanoparticles in the cytoplasm of living cells
I. Alieva – Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia
I. Kireev – Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia
A. Rakhmanina – L. F. Vereshchagin Institute for High Pressure Physics of the RAS, Troitsk, Russia
A. Garanina – Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia
O. Strelkova – Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia
O. Zhironkina – Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia
V. Cherepaninets – Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia
V. Davydov – L. F. Vereshchagin Institute for High Pressure Physics of the RAS, Troitsk, Russia
V. Khabashesku – Center for Technology Innovation, Baker Hughes Inc., Houston, TX, 77040, USA
V. Agafonov – GREMAN, UMR CNRS 7347, Université Francois Rabelais, 37200 Tours, France; viatcheslav.agafonov@univ-tours.fr
R. Uzbekov – Faculté de Médecine, Université Francois Rabelais, Tours, France; Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, Russia; rustem.uzbekov@univ-tours.fr
The uptake of superparamagnetic Fe7C3@C nanoparticles into living cells and their behavior once inside the cell was investigated. The cells used were shown to absorb the nanoparticle aggregates over the first 30 minutes. After absorption, these aggregates moved towards the center of the cell and accumulated near the cell nucleus. No toxic effects on cell physiology were observed. In a magnetic field, the particles aligned in the cells along magnetic lines and shifted to the magnet’s side. During long-term cultivation, Fe7C3@C nanoparticle aggregates were ultimately discarded via exocytosis.
Keywords: superparamagnetic Fe7C3@C nanoparticles, living cells, electron microscopy, magnetic field.
PACS 81.40.Rs, 81.07.b, 87.18.h, 87.16.Wd, 87.17.Rt, 87.64.Ee, 87.64.M-, 87.64.mh, 87.85.jj
DOI 10.17586/2220-8054-2016-7-1-158-160
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