NANOSYSTEMS: PHYSICS, CHEMISTRY, MATHEMATICS, 2018, 9 (3), P. 430–438
Cytotoxicity analysis of gadolinium doped cerium oxide nanoparticles on human mesenchymal stem cells
A. L. Popov – Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia
I.V. Savintseva – Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia
E. A. Mysina – Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia
A. B. Shcherbakov – Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv D0368, Ukraine
N. R. Popova – Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia
O. S. Ivanova – Kurnakov Institute of General and Inorganic Chemistry of Russian Academy of Sciences, Moscow 119991, Russia
D. D. Kolmanovich – Kabardino-Balkarian State University, Nalchik, 360004, Russia
V. K. Ivanov – Kurnakov Institute of General and Inorganic Chemistry of Russian Academy of Sciences, Moscow 119991; Moscow Technological University, Institute of Fine Chemical Technologies, Moscow 119991, Russia; van@igic.ras.ru
A complex analysis of cytotoxicity was performed for a nanodispersed sol of cerium dioxide doped with gadolinium (Ce0.8Gd0.2O2-x) using a culture of mesenchymal stem cells. The absence of cyto- and genotoxicity over a wide range of concentrations (10-5 – 10-9 M) was demonstrated. At that, the highest concentration of Ce0.8Gd0.2O2-x nanoparticles (10-4 M) was found to slightly reduce the activity of intracellular dehydrogenase, yet not leading to the development of apoptosis and further cell death. The obtained results confirm a high degree of Ce0.8Gd0.2O2-x nanoparticle biocompatibility, which opens prospects for their safe application as a contrasting agent in the magnetic resonance tomography.
Keywords: ceria, gadolinium, nanomaterials, cytotoxicity, mesenchymal stem cells.
PACS 87.55.ne, 87.85.Rs, 81.07.Bc
DOI 10.17586/2220-8054-2018-9-3-430-438
